{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:53:13Z","timestamp":1760608393858},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5319,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In a previous study we have shown that synovial fluid mononuclear cells from many rheumatoid arthritis (RA) patients exhibit an enhanced response to <jats:italic>M. tuberculosis<\/jats:italic> antigens as compared to peripheral blood mononuclear cells. The 65\u2010kDa heat\u2010shock protein of <jats:italic>M. tuberculosis<\/jats:italic> was shown not to play an important role in this response, therefore other mycobacterial proteins must be involved. In this study we have investigated the possibility that synovial fluid T cells from RA patients predominantly recognize a limited number of <jats:italic>M. tuberculosis<\/jats:italic> antigens, as a result of a lesion\u2010specific activation of only those <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cells that have cross\u2010reacted with joint\u2010related autoantigens. From the synovial fluid of four RA patients <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cell clones were isolated and analyzed for their phenotype, HLA\u2010DR restriction and proliferation to immunoblot fractions containing sodium dodecyl sulfate\u2010polyacrylamide gel\u2010separated <jats:italic>M. tuberculosis<\/jats:italic> proteins of known molecular weight range. The overall <jats:italic>M. tuberculosis<\/jats:italic> immunoblot recognition pattern of the clones was strikingly heterogeneous. Within a panel of 15 clones 12 different antigenic specificities could be distinguished. In other words, we did not observe a dominant recognition of a few <jats:italic>M. tuberculosis<\/jats:italic> antigens by synovial fluid T cells. This argues against the hypothesis that the elevated synovial T cell reactivity against <jats:italic>M. tuberculosis<\/jats:italic> is a reflection of an <jats:italic>in vivo<\/jats:italic> expansion of a limited number of different types of <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cells as a result of a cross\u2010reaction with putative joint autoantigens.<\/jats:p>","DOI":"10.1002\/eji.1830210530","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T12:50:04Z","timestamp":1172753404000},"page":"1297-1302","source":"Crossref","is-referenced-by-count":19,"title":["Diversity in antigen recognition by <i>Mycobacterium tuberculosis<\/i>\u2010reactive T cell clones from the synovial fluid of rheumatoid arthritis patients"],"prefix":"10.1002","volume":"21","author":[{"given":"Pieter C. M.","family":"Res","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela L. 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