{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T05:42:09Z","timestamp":1698471729294},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5227,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Benign monoclonal gammapathy (BMG) is defined as a benign monoclonal B cell proliferative disorder characterized by the presence of a persisting component of homogenous immunoglobulins (H\u2010Ig) in the serum. A possible role of antigenic stimulation in the development of BMG has been suggested. From a C57BL mouse, a murine model for BMG, we have Isolated clonally related B cells in order to investigate the occurrence of somatic mutations in the variable heavy chain (V<jats:sub>H<\/jats:sub>) region of the genes of H\u2010Ig\u2010producing B cell clones. Therefore, B cells were immortalized by hybridoma technology. The hybridomas were screened for resemblance of the serum H\u2010Ig component by Wieme agar electrophoresis, followed by immunoblotting and isoelectrofocusing. Clonal relationship was investigated by Southern blot analysis using a J<jats:sub>H<\/jats:sub> probe. In this way we isolated five hybridomas producing an IgG<jats:sub>2a,<jats:italic>x<\/jats:italic><\/jats:sub> that was identical to the original serum H\u2010Ig component according to testing with anti\u2010idiotypic antisera. mRNA sequencing of four hybridomas showed only one base pair difference in the V<jats:sub>H<\/jats:sub> genes. This particular gene belonged to the J558 V<jats:sub>H<\/jats:sub> gene family. When compared to the most closely related known V<jats:sub>H<\/jats:sub> sequence, three base pair differences were found. The almost complete absence of base pair differences in the V<jats:sub>H<\/jats:sub> genes of the four sequenced hybridomas, compared with an independently derived hybridoma, suggests that the same germ\u2010line V<jats:sub>H<\/jats:sub> gene has been used and that somatic mutations were infrequent in our BMG clone.<\/jats:p>","DOI":"10.1002\/eji.1830210817","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:02:33Z","timestamp":1172754153000},"page":"1893-1898","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and molecular characterization of the B cells producing the paraprotein in a case of benign monoclonal gammapathy in C57BL mice"],"prefix":"10.1002","volume":"21","author":[{"given":"Nicolaas A.","family":"Bos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia G.","family":"Meeuwsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"De Glopper\u2010Van der Veer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodoor W.","family":"Van den Akker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiri","family":"Radl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kornelisje A.","family":"Zwaagstra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robbert","family":"Benner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0954-6820.1984.tb05032.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-00377-5.50006-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-71562-4_32"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(80)90119-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000234667"},{"key":"e_1_2_1_7_2","first-page":"229","volume-title":"Monoclonal gammapathies II\u2010Clinical Significance and Basic Mechanisms, Topics in Aging Research in Europe","author":"Radl J.","year":"1989"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/328805a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-442702-0.50020-0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830191223"},{"key":"e_1_2_1_11_2","unstructured":"Williams K. 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