{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T06:52:40Z","timestamp":1742799160651},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5196,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>B cell differentiation requires adhesion of B cell progenitors to bone marrow (BM) or fetal liver stroma. We show that B lymphoid cells can adhere to the BM stroma cell line CS 1.3, <jats:italic>in vitro.<\/jats:italic> Two monoclonal antibodies, SAB\u20101 and SAB\u20102, inhibited the adhesion of a B220<jats:sup>+<\/jats:sup> progenitor B cell line but did not interfere with the binding of cytoplasmic \u03bc\u2010chain\u2010positive pre\u2010B cells or mature B cells to the BM stromal cell line. Injection of both SAB\u20101 and SAB\u20102 antibodies into pregnant mice reduced by 90% the number of B220<jats:sup>+<\/jats:sup> B lineage cells in the livers of their embryos. Livers from such embryos also were virtually devoid of cells able to give rise to B cell colonies in soft agar cultures (CFU\u2010preB). Either antibody separately had no effect. Flow cytometry analysis show that SAB\u20101 is present on CS 1.3 stroma cells and on a pre\u2010B cell line while SAB\u20102 is present on pro\u2010B and pre\u2010B cell lines, but not on CS 1.3 stromal cells. SAB\u20101 and SAB\u20102 react with different molecules and neither antibody seems to recognize CD44, and adhesion molecule that may also participate in B cell differentiation. Proteinase K and trypsin can digest both SAB\u20101 and SAB\u20102 antigens from viable cells suggesting that both are cell surface proteins. We propose that antibodies SAB\u20101 and SAB\u20102 probably recognize novel cell\u2010cell adhesion molecules, and that these molecules are involved in the interactions between B cell progenitors and stroma cells.<\/jats:p>","DOI":"10.1002\/eji.1830210911","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:01:49Z","timestamp":1172754109000},"page":"2043-2049","source":"Crossref","is-referenced-by-count":7,"title":["Monoclonal antibodies that block adhesion of B cell progenitors to bone marrow stroma <i>in vitro<\/i> prevent B cell differentiation <i>in vivo<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Beat A.","family":"Imhof","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Schlienger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Handloser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Hesse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Slanicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Gisler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.3.513.513"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(89)90058-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90620-X"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90230-C"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/346425a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.11.2199285"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2466335"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90636-3"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/331624a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1002\/j.1460-2075.1989.tb08437.x","volume":"8","author":"Horley K. 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