{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:41:54Z","timestamp":1761100914943},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5196,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have studied the lymphocytic choriomeningitis virus (LCMV)\u2010specific cytotoxic T cell response in transgenic mice expressing either the T cell receptor (TcR) \u03b1 (V<jats:sub>\u03b1<\/jats:sub>2\/J<jats:sub>\u03b1<\/jats:sub>TA31) or the corresponding TcR \u03b2 (V<jats:sub>\u03b2<\/jats:sub>8.1\/D<jats:sub>\u03b2<\/jats:sub>\/J<jats:sub>\u03b2<\/jats:sub>2.4) chain originally isolated from the LCMV glyconrotein specific (residues 32\u201342), H\u20102D<jats:sup>b<\/jats:sup>\u2010restricted T cell clone P14. The expression of single transgenic TcR chains did not influence the corresponding endogenous TcR V gene usage in unstimulated T cells indicating that one particular TcR \u03b1 or \u03b2 chain can randomly pair with different V<jats:sub>\u03b2<\/jats:sub> or V<jats:sub>\u03b1<\/jats:sub> chains without any obvious bias. However, upon infection with LCMV, reactive cytotoxic T lymphocytes (CTL) from P14 \u03b2\u2010transgenic mice were predominantly V\u03b12<jats:sup>+<\/jats:sup> whereas CTL from P14 \u03b1\u2010transgenic mice preferentially expressed V<jats:sub>\u03b2<\/jats:sub>8.1 and unexpectedly also V<jats:sub>\u03b2<\/jats:sub>8.3 (but not V<jats:sub>\u03b2<\/jats:sub>8.2). Correspondingly the LCMV\u2010specific CTL response in both \u03b1 and \u03b2 TcR\u2010transgenic mice was strongly biased to the original P14 T cell epitope (LCMV glycoprotein residues 32\u201342). Sequence analysis of a large panel of LCMV\u2010reactive \u201chalf\u2010transgenic\u201d TcR from P14 single receptor chain\u2010transgenic mice revealed a highly conserved VJ<jats:sub>\u03b1<\/jats:sub> and a more diverse VDJ<jats:sub>\u03b2<\/jats:sub> junctional region. This report demonstrates that the antigen specificity of the studied TcR depends on the specific combination of both TcR \u03b1 and \u03b2 chains which implies that amino acids located in the TcR V<jats:sub>\u03b1<\/jats:sub> and V<jats:sub>\u03b2<\/jats:sub> segments as well as in the junctional region are involved in binding of the viral antigenic fragment.<\/jats:p>","DOI":"10.1002\/eji.1830210930","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:01:49Z","timestamp":1172754109000},"page":"2195-2202","source":"Crossref","is-referenced-by-count":54,"title":["Involvement of both T cell receptor V<sub>\u03b1<\/sub> and V<sub>\u03b2<\/sub> variable region domains and \u03b1 chain junctional region in viral antigen recognition"],"prefix":"10.1002","volume":"21","author":[{"given":"Daniel","family":"Br\u00e4ndle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"B\u00fcrki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie A.","family":"Wallace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urs Hoffmann","family":"Rohrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tak W.","family":"Mak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Hengartner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/248701a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/329506a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/329512a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3317824"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.05.040187.003101"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/320232a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/325125a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/334395a0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/321219a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/324679a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90558-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90079-7"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"4851","DOI":"10.4049\/jimmunol.144.12.4851","volume":"144","author":"Lai M.\u2010Z.","year":"1990","journal-title":"J. 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