{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T21:08:32Z","timestamp":1782335312871,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":5009,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In \u03bc 17.2.25\u2010transgenic (M54) mice the absolute number of surface IgM (slgM) B cells in lymphoid organs is drastically reduced compared to normal C57BL\/6 mice and a high frequency of B cells express the immunoglobulin (Ig) encoded by the transgene rather than endogenous Ig on the surface. To determine the effect of a \u03bc trangene on B cell development, adoptive cell transfers were performed using \u03bc transgenic (M54) bone marrow and fetal liver cells. The data presented support the following conclusions: (a) adult transgenic bone marrow contains functional B cell precursors able to mature and repopulate the spleen and peritoneum of recipient mice. The relative frequency of transgene (sIgM<jats:sup>a<\/jats:sup>) and endogenous (sIgM<jats:sup>b<\/jats:sup>) surface sIgM\u2010positive B cells reconstituted by transgenic bone marrow in allotype\u2010matched C57BL\/6 recipients is the same as in the M54 donors; (b) serum analysis indicates that transgenic bone marrow donor cells can reconstitute B cells in congenic and severe combined immunodeficient (SCID) recipient mice; (c) transgenic fetal liver cells are not a richer source of precursors for B cells expressing endogeneous Ig; (d) in transgenic mice sIgM<jats:sup>+<\/jats:sup> B cells are not restricted to the CD5<jats:sup>+<\/jats:sup> phenotype, however, the relative frequency of sIgM<jats:sup>b<\/jats:sup> B cells that are CD5<jats:sup>+<\/jats:sup> is higher in transgenic than normal mice; and (e) bone marrow cells from adult normal and transgenic mice are able to generate CD5<jats:sup>+<\/jats:sup> B lymphocytes in the spleen and peritoneum of allotype\u2010congenic and neonatal SCID recipient mice.<\/jats:p><jats:p>The results indicate that the presence of a complete \u03bc heavy chain transgene does not result in a selective developmental block of \u201cconventional\u201d bone marrow\u2010derived pre\u2010B and B cells.<\/jats:p>","DOI":"10.1002\/eji.1830220318","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:55:58Z","timestamp":1172757358000},"page":"745-751","source":"Crossref","is-referenced-by-count":16,"title":["The effect of an immunoglobulin \u03bc transgene on B cell maturation"],"prefix":"10.1002","volume":"22","author":[{"given":"John","family":"Iacomini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thereza","family":"Imanishi\u2010Kari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-354865-8.50021-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90259-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80107-0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/3.2.185"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200507"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200506"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90389-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/3.1.67"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3975629"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.2.589"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1986.tb01163.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160116"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170315"},{"key":"e_1_2_1_15_2","first-page":"1","volume-title":"Chemical Immunology","author":"Kearney J. 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