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We now report the results of a more comprehensive analysis of memory T cell function, in old and young mice, to non\u2010cognate activators (Con A and the staphylococcal enterotoxin SEB). We report that memory T cells, isolated by removing cells bearing the CD45RB determinant, contain very few cells able to respond to either Con A or SEB under limiting dilution culture conditions, whether the responses are measured by IL\u20102 or by IL\u20103 accumulation. As a control, we show that memory T cells do respond strongly, at limiting dilution, to recently encountered priming antigens, <jats:italic>i.e. Schistosoma mansoni<\/jats:italic> egg antigen; the limiting dilution culture protocol thus does not preclude activation of memory T cells when cognate stimuli are presented to antigen\u2010specific cells. These data suggest that virgin and memory T cells may differ fundamentally in their activation requirements, and suggest further that the accumulation, with age, of memory T cells accounts for the low responsiveness of old mice to non\u2010cognate mitogens.<\/jats:p>","DOI":"10.1002\/eji.1830220408","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T14:35:32Z","timestamp":1172759732000},"page":"931-935","source":"Crossref","is-referenced-by-count":69,"title":["Memory T lymphocyte hyporesponsiveness to non\u2010cognate stimuli: a key factor in age\u2010related immunodeficiency"],"prefix":"10.1002","volume":"22","author":[{"given":"Kevin","family":"Flurkey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Stadecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61527-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60655-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(88)90106-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.4049\/jimmunol.132.1.63","volume":"132","author":"Miller R. 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