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An <jats:italic>in vitro<\/jats:italic> manifestation of this condition is the virtual inability of CBA\/N B cells to form colonies in lipopolysaccharide (LPS)\u2010containing semisolid agar cultures. In this report we show evidence that the colony\u2010forming ability of CBA\/N spleen cells can be effectively restored by the bone marrow stromalderived cell line S17. Spleen cells from 4\u20135\u2010week\u2010old homozygous CBA\/N female mice were grown in double\u2010layer agar cultures containing S17 feeder layers. Control cultures contained the fibroblast\u2010like cell line 95.17 or were treated with medium alone. It was found that at an input cell concentration of 10<jats:sup>4<\/jats:sup> cells per plate, CBA\/N colony formation was increased from a frequency of approximately 1 in 5000 to 1 in 50 total splenic cells. Studies with purified surface immunoglobulin\u2010positive cells indicate the direct involvement of S17 in this process. The CBA\/N colonies formed were dependent on the presence of a mitogen (LPS) and secreted detectable amounts of IgM. Major implications of these findings and the application of this assay system to study the CBA\/N defect have been discussed.<\/jats:p>","DOI":"10.1002\/eji.1830220419","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T14:35:32Z","timestamp":1172759732000},"page":"1001-1006","source":"Crossref","is-referenced-by-count":4,"title":["The stromal cell line S17 supports the growth of lipopolysaccharide\u2010stimulated CBA\/N spleen cell colonies <i>in vitro<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Arumugavadivel","family":"Narendran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Cumano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Dorshkind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Paige","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.136.4.931"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60834-2"},{"key":"e_1_2_1_4_2","first-page":"81","volume":"41","author":"Kincade P. 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