{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T11:07:07Z","timestamp":1770721627910,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":4917,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cutaneous leishmaniasis is initiated by the bite of an infected sandfly and inoculation of <jats:italic>Leishmania major<\/jats:italic> parasites into the mammalian skin. Macrophages are knownto play a central role in the course of infection because they are the prime host cellsand function as antigen\u2010presenting cells (APC) for induction of the cell\u2010mediated immune response. However, in addition to macrophages in the dermis, the skin contains epidermal Langerhans cells (LC) which can present antigen (Ag) to T cells. Therefore, using a murine model of cutaneous leishmaniasis, we analyzed the ability of epidermal cells to induce a T cell response to <jats:italic>L.major<\/jats:italic>. The results demonstrated that freshly isolated LC, but not cultured LC, are highly active in presenting <jats:italic>L.major<\/jats:italic> Ag <jats:italic>in vitro<\/jats:italic> to T cells from primed mice and toa <jats:italic>L.major<\/jats:italic>\u2010specific T cell clone. Furthermore, freshly isolated LC had theability to retain <jats:italic>L.major<\/jats:italic> Ag in immunogenic form for at least 2 days. Their efficiency was much greater than that of irradiated spleen cells, a standard population of APC. LC stimulated both Tcell proliferation and production of the lymphokines interleukin (IL)\u20102 and IL\u20104. The response was Ag specific and could be induced by lysate of <jats:italic>L.major<\/jats:italic> parasites and by live organisms. The data suggest that epidermal LC are important APC in cutaneous leishmaniasis. They may perform a critical function by capturing <jats:italic>L.major<\/jats:italic> Ag in the skin and presenting it either to quiescent T cells circulating through the draining lymph node or locally to T effector cells infiltrating the cutaneous lesion.<\/jats:p>","DOI":"10.1002\/eji.1830220603","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:19:30Z","timestamp":1172773170000},"page":"1341-1347","source":"Crossref","is-referenced-by-count":37,"title":["Murine epidermal Langerhans cells are potent stimulators of an antigen\u2010specific T cell response to <i>Leishmania major<\/i>, the cause of cutaneous leishmaniasis"],"prefix":"10.1002","volume":"22","author":[{"given":"Antje","family":"Will","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Blank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"R\u00f6llinghoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidrun","family":"Moll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"342","volume":"44","author":"Berman J. 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