{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T15:29:59Z","timestamp":1698161399959},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":4906,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conjugate formation between cytotoxic T lymphocytes (CTL) and target B cells, as observed <jats:italic>in vitro<\/jats:italic>, is mediated by interactions between adhesion molecules on the two cell surfaces rather than involving immune recognition through the T cell receptor. It is still not clear to what extent such adhesive contacts facilitate the process of immune recognition and target cell lysis. However, work on the Epstein\u2010Barr virus (EBV)\u2010associated malignancy Burkitt's lymphoma (BL) has suggested that down\u2010regulation of one particular adhesion molecule, the lymphocyte function\u2010associated antigen LFA\u20103, on the tumor cell surface is a key factor in allowing these target cells to escape EBV\u2010speficic T cell surveillance. To examine this directly, we used a cDNA for the full\u2010length transmembrane form of LFA\u20103 to construct a recombinant vaccinia virus (Vacc\u2010LFA 3), which is capable of restoring surface LFA\u20103 in adhesion molecule\u2010negative BL cell lines to levels as high as seen in EBV\u2010transformed lymphoblastoid cell lines (LCL); biochemical studies confirmed expression of the authentic N\u2010glycosylated protein. The recombinant vaccinia\u2010encoded LFA\u20103 was functional as an adhesion molecule since BL cells acutely infected with Vacc\u2010LFA\u20103 then acquired the ability to form conjugates with activated T cells <jats:italic>in vitro.<\/jats:italic> However, there was no clear dependence upon LFA\u20103 when such BL cell lines were tested as targets for cytotoxic T lymphocytes (CTL). Firstly, LFA\u20103<jats:sup>\u2212<\/jats:sup> BL cells could be killed by allospecific CTL recognizing HLA class I alloantigens, in some cases as efficiently as the corresponding LCL. In other cases where lysis was slightly below that of the LCL, Vacc\u2010LFA\u20103 infection of the BL cells increased lysis up to, but never beyond, LCL values. Secondly, we studied the sensitivity of BL to EBV\u2010specific HLA class I\u2010restricted CTL using a BL target line which was LFA\u20103<jats:sup>\u2212<\/jats:sup> but which expressed the same spectrum of EBV target proteins as an LCL. This line was not recognized by appropriately HLA\u2010matched effectors, even after restoration of LFA\u20103 expression. We conclude that the LFA\u20103 status of BL cells influences their conjugate forming ability in <jats:italic>in vitro<\/jats:italic> assays but not necessarily their sensitivity to immune T cell\u2010mediated cytolysis.<\/jats:p>","DOI":"10.1002\/eji.1830220712","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T16:29:52Z","timestamp":1172766592000},"page":"1741-1748","source":"Crossref","is-referenced-by-count":3,"title":["Restoration of the LFA\u20103 adhesion pathway in Burkitt's lymphoma cells using an LFA\u20103 recombinant vaccinia virus: consequences for T cell recognition"],"prefix":"10.1002","volume":"22","author":[{"given":"Heather","family":"Griffin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill","family":"Brooks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Rickinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/329512a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/334395a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/346425a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/323262a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.4049\/jimmunol.139.4.1037","volume":"139","author":"Shaw S.","year":"1987","journal-title":"J. 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