{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T17:57:32Z","timestamp":1722880652379},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4845,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,9]]},"abstract":"Abstract<\/jats:title>The aim of this study was to determine whether synovial fluid (SF) T cells in patients with juvenile rheumatoid arthritis (JRA) are restricted in their T cell receptor (TcR) gene repertoire. The quantitative polymerase chain reaction (QPCR) was used to compare the transcription of V\u03b2<\/jats:sub> and V\u03b1<\/jats:sub> gene families in freshly isolated SF T cells, in interleukin\u20102 receptor\u2010positive (IL\u20102R+<\/jats:sup>) T cells and in peripheral blood (PB) T cells from 18 patients. Significantly less V\u03b2<\/jats:sub> families are detected in SF when compared with PB (p<\/jats:italic> > 0.0003). The TcR V\u03b2<\/jats:sub> gene usage by IL\u20102R+<\/jats:sup> T cells was even less heterogeneous when compared with freshly isolated SF T cells (p<\/jats:italic> > 0.0002). Freshly isolated SF T cells from the left and the right knees of four patients transcribed the same V\u03b2<\/jats:sub> families. Furthermore, we demonstrate that in SF the distribution of certain TcR V\u03b2<\/jats:sub> gene segments in CD4+<\/jats:sup> and CD8+<\/jats:sup> T cells differed from that in PB of the same patient.<\/jats:p>The TcR V\u03b1<\/jats:sub> usage was studied in IL\u20102R+<\/jats:sup> T cells from six patients who had shown restriction in their SF TcR V\u03b2<\/jats:sub> gene usage. Only two to five TcR \u03b1 transcripts were detected in three of these patients while a broad TcR V\u03b1<\/jats:sub> usage was seen in the other three patients. Sequence analysis of the SF V\u03b2<\/jats:sub> 20 cDNA clones generated from the IL\u20102R+<\/jats:sup> T cells of two patients demonstrated an oligoclonal expansion. Taken together, our data could indicate an antigen\u2010 and\/or superantigen\u2010driven expansion of selected T cells in the synovial compartment.<\/jats:p>","DOI":"10.1002\/eji.1830220934","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T17:49:44Z","timestamp":1172771384000},"page":"2413-2418","source":"Crossref","is-referenced-by-count":31,"title":["Limited heterogeneity of T cell receptor variable region gene usage in juvenile rheumatoid arthritis synovial T cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Mouldy","family":"Sioud","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Kjeldsen\u2010Kragh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sel","family":"Suleyman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odd","family":"Vinje","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob B.","family":"Natvig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8ystein","family":"F\u00f8rre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199002050-00019"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1982.tb00643.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.4049\/jimmunol.133.3.1230","volume":"133","author":"Burmester G. 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