{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T23:18:08Z","timestamp":1770765488010,"version":"3.50.0"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":4795,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human V<jats:sub>\u03b2<\/jats:sub> (hV<jats:sub>\u03b2<\/jats:sub>) regions have been expressed in the context of mouse T cell receptor (TcR)\u2010CD3 complexes, and subsequently used to raise hV<jats:sub>\u03b2<\/jats:sub>\u2010specific monoclonal antibodies (mAb). The method of expression of hV<jats:sub>\u03b2<\/jats:sub> outlined in this report contrasts in its versatility with the one reported by Choi et al. (<jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic> 1991. <jats:italic>88:<\/jats:italic> 8357). For instance, we have applied it successfully to the construction of mouse T cell transfectants expressing hV<jats:sub>\u03b2<\/jats:sub>1, hV<jats:sub>\u03b2<\/jats:sub>2, hV<jats:sub>\u03b2<\/jats:sub>3, hV<jats:sub>\u03b2<\/jats:sub>8, hV<jats:sub>\u03b2<\/jats:sub>9, hV<jats:sub>\u03b2<\/jats:sub>13.5, hV<jats:sub>\u03b2<\/jats:sub>19, hV<jats:sub>\u03b2<\/jats:sub>21, and hV<jats:sub>\u03b2<\/jats:sub>22 gene segments. mAb against the hV<jats:sub>\u03b2<\/jats:sub>2 and hV<jats:sub>\u03b2<\/jats:sub>19 regions have been raised by using these transfectants as immunogens in mice. Here, we illustrate the application of the anti\u2010hV<jats:sub>\u03b2<\/jats:sub> mAb to the measurement of human T cells that react with the staphylococcal toxic\u2010shock syndrome toxin\u20101.<\/jats:p>","DOI":"10.1002\/eji.1830221043","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:13:36Z","timestamp":1172754816000},"page":"2749-2752","source":"Crossref","is-referenced-by-count":22,"title":["A versatile method to produce antibodies to human T cell receptor V<sub>\u03b2<\/sub> segments: frequency determination of human V<sub>\u03b2<\/sub>2<sup>+<\/sup> T cells that react with toxic\u2010shock syndrome toxin\u20101"],"prefix":"10.1002","volume":"22","author":[{"given":"Fran\u00e7ois","family":"Romagn\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Besnardeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8941"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2908"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210920"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.19.8357"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830191214"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.123.4.1548"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.3.765"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220319"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90444-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00736.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.14.4831"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120908"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.131.4.1789"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.5.1374"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2524876"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.3.981"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"4360","DOI":"10.4049\/jimmunol.147.12.4360","volume":"147","author":"Plaza A.","year":"1991","journal-title":"J. Immunol."}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830221043","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830221043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:23:00Z","timestamp":1698056580000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830221043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":17,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1992,10]]}},"alternative-id":["10.1002\/eji.1830221043"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830221043","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}