{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T20:25:32Z","timestamp":1698438332886},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4692,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Young (H\u20102<jats:sup>d<\/jats:sup>, L<jats:sup>d+<\/jats:sup>) severe combined immunodeficiency (scid) mice were injected intravenously with 10<jats:sup>5<\/jats:sup> CD4+CD8<jats:sup>\u2212<\/jats:sup> T cells purified from spleen, thymus or lymph nodes (LN) of dm2 (H\u20102<jats:sup>d<\/jats:sup>, L<jats:sup>d<\/jats:sup> ) donor mice. In the immunodeficient recipients, the lymphoid compartment in the splenic white pulp was repopulated with donor\u2010type T cells and cellularity in the red pulp was increased. In addition, donor\u2010type CD4<jats:sup>+<\/jats:sup> T cells repopulated the peritoneal cavity, mesenteric LN and the lamina propria of the small intestine of scid mice, but were undetectable in thymus and peripheral (inguinal, axillary) LN. Histological examination of repopulated mesenteric LN showed expanded subcapsular sinuses, repopulated cortical areas, but poorly developed high endothelial venules (HEV) indicating deficient blood\u2010LN lymphocyte recirculation. The engrafted CD4<jats:sup>+<\/jats:sup> T cell population had the surface phenotype of memory T cells (CD44\/Pgp\u2010l<jats:sup>high<\/jats:sup> CD45RB<jats:sup>low<\/jats:sup>) and expressed the Peyer's patch HEV\u2010specific homing receptor CD49d (LPAM\u20101), but not the LN HEV\u2010specific homing receptor LECAM\u2010l. The CD4+ T cell population in spleen and mesenteric LN of transplanted scid mice displayed a diverse T cell receptor\u2010V(3 repertoire. Transfer of titrated numbers (10<jats:sup>3<\/jats:sup>, 10<jats:sup>4<\/jats:sup>, 10<jats:sup>5<\/jats:sup> cells per mouse) of CD4<jats:sup>+<\/jats:sup> T cells into scid mice established donor\u2010type T cell populations with this unusual homing pattern in all recipients. Repeated serial transfers of dm2 CD4<jats:sup>+<\/jats:sup> T cells through young scid mice revealed an extensive <jats:italic>in vivo<\/jats:italic> expansion potential of transferred cells for &gt; 18 months. The experimental system described represents an <jats:italic>in vivo<\/jats:italic> model to study the functional competence and the differentiation potential of a murine memory CD4<jats:sup>+<\/jats:sup> T cell subset.<\/jats:p>","DOI":"10.1002\/eji.1830230208","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:53:06Z","timestamp":1172775186000},"page":"350-356","source":"Crossref","is-referenced-by-count":18,"title":["Selective engraftment of memory CD4<sup>+<\/sup> T cells with an unusual recirculation pattern and a diverse T cell receptor\u2010V\u03b2 repertoire into scid mice"],"prefix":"10.1002","volume":"23","author":[{"given":"J\u00f6rg","family":"Reimann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Rudoiphi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Tscherning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mogens H.","family":"Claesson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1991.tb00617.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.001543"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.002203"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/344742a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90448-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/350423a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90030-G"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90029-C"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2730"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.002531"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210240"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210704"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/3.7.657"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210913"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1991.tb01604.x"},{"key":"e_1_2_1_17_2","unstructured":"Rudolphi A. 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