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After confirming the semiquantitative and reproducible nature of the A\u2010PCR technique, we assessed the complete V\u03b2 repertoire in sorted CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> lymphocyte populations from three normal donors. These experiments confirmed the absence of V\u03b2\u2010restricted deletions in human peripheral cells, in contrast to several mouse strains. This feature makes it difficult to study negative selection in man, given the apparent absence of an endogenous superantigen corresponding to the Mis system in the mouse. To investigate human V\u03b2 repertoire shaping, we studied VP usage in CD4+ and CD8<jats:sup>+<\/jats:sup> T cells from children with an inherited immunodeficiency characterized by defective expression of human leukocyte antigen class II molecules. An initial study using anti\u2010V\u03b2 monoclonal antibodies failed to show significant abnormalities in V\u03b2 usage. Four patients analyzed using the A\u2010PCR method all had a polyclonal V\u03b2 repertoire, suggesting normal positive selection and raising questions as to the importance of VP major histocompatibility complex (MHC) interactions and the role of thymic MHC density in shaping the V\u03b2 repertoire.<\/jats:p>","DOI":"10.1002\/eji.1830230425","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:44:25Z","timestamp":1172774665000},"page":"928-934","source":"Crossref","is-referenced-by-count":32,"title":["Normal T cell receptor V\u03b2 usage in a primary immunodeficiency associated with HLA class II deficiency"],"prefix":"10.1002","volume":"23","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Rieux\u2010Laucat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franchise","family":"le Deist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franchise","family":"Selz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain Fischer","family":"And","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"de Villartay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/333742a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00736.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1972592"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.002531"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90501-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/355224a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.3.639"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.003525"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.4049\/jimmunol.146.12.4392","volume":"146","author":"Robinson M. 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