{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:29:38Z","timestamp":1790184578081,"version":"4.1.0"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4572,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1993,6]]},"abstract":"Abstract<\/jats:title>The surrogate light chain encoded by the two pre\u2010B cell\u2010specific genes VpreB<\/jats:sub> and \u03bb5<\/jats:sub> plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the \u03bb 5<\/jats:sub>gene results in a depletion of B220+<\/jats:sup> CD43\u2212<\/jats:sup> IgM\u2212<\/jats:sup> pre\u2010B cells in bone marrow, and in a delayed appearance both of CD5+<\/jats:sup> as well as CD5\u2212<\/jats:sup> surface immunoglobulin (slg)+<\/jats:sup> B cells in the periphery. In this report we show that DH<\/jats:sub>JH<\/jats:sub>\u2010rearranged B220\u2212<\/jats:sup> and B220+<\/jats:sup>, CD43+<\/jats:sup>, c\u2010kit+<\/jats:sup>, sIgM\u2212<\/jats:sup> pro\u2010 and pre\u2010B\u2010I cells with long\u2010term capacity to proliferate in vitro<\/jats:italic> on stromal cells in the presence of interleukin\u20107 are present in normal numbers in the bone marrow of \u03bb 5<\/jats:sub>T\/\u03bb.5<\/jats:sub>T mice at various ages. They express normal levels of VpreB<\/jats:sub> mRNA but, in contrast to normal pre\u2010B\u2010I cells, do not express surrogate light chain on their surface. Pre\u2010B\u2010I cells from fetal liver and bone marrow of \u03bb 5<\/jats:sub>T\/\u03bb 5<\/jats:sub>T mice differentiate with normal kinetics and in normal numbers to sIg+<\/jats:sup>, mitogen\u2010reactive B cells. These results suggest that the delayed generation of sIg+<\/jats:sup> B cells in the peripheral, mature compartments of CD5+<\/jats:sup> and CD5\u2212<\/jats:sup> cells could be accounted for by the daily production of approximately 5 \u00d7 105<\/jats:sup> sIg+<\/jats:sup> B cells from the pre\u2010B\u2010I cell pool in the absence of a normal pool of pre\u2010B\u2010II cells.<\/jats:p>","DOI":"10.1002\/eji.1830230614","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:05:38Z","timestamp":1172775938000},"page":"1284-1288","source":"Crossref","is-referenced-by-count":69,"title":["B cell development in mice with a defective \u03bb5<\/sub> gene"],"prefix":"10.1002","volume":"23","author":[{"given":"Antonius","family":"Rolink","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Karasuyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Grawunder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Haasner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Kudo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fritz","family":"MeLchers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90060-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3317825"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1989.054.01.023"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90293-L"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/j.1460-2075.1991.tb07953.x","volume":"10","author":"Rolink A.","year":"1991","journal-title":"EMBO J."},{"key":"e_1_2_1_7_2","unstructured":"Rolink A. 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