{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T13:10:48Z","timestamp":1698412248772},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":4420,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In HPB\u2010ALL T\u2010cells the p59<jats:sup>fyn<\/jats:sup> tyrosine kinase is regulated by the CD45 phosphotyrosine phosphatase and plays a critical role in coupling the T cell receptor (TCR) to the generation of intracellular signals which include diacylglycerol (DAG) production and protein kinase C activation. The aim of this study was to determine the phospholipid pools from which the DAG is generated and to identify which phospholipase activities are regulated by the TCR. When CD45<jats:sup>+<\/jats:sup>cells were pre\u2010labeled with [<jats:sup>3<\/jats:sup>H] arachidonic acid, CD3\u2010antigen cross\u2010linking stimulated negligible increases in both [<jats:sup>3<\/jats:sup>H]DAG and [<jats:sup>3<\/jats:sup>H] phosphatidic acid (PA). However, CD3 monoclonal antibody (mAb) induced an increase of 300 % in [<jats:sup>3<\/jats:sup>H]PA when the cells were permeabilized with streptolysin\u2010O, and this correlated with increased levels of protein tyrosine phosphorylation. Stimulation of [<jats:sup>3<\/jats:sup>H] PA production upon CD3 cross\u2010linking was 77 % lower in permeabilized CD45<jats:sup>\u2212<\/jats:sup> cells than in CD45<jats:sup>+<\/jats:sup> cells, consistent with the reduced activity of p59<jats:sup>fyn<\/jats:sup> in CD45<jats:sup>\u2212<\/jats:sup> cells. The stimulated production of PA was not mediated by activation of phospholipase D (PLD), although the presence of a G\u2010protein\u2010regulated PLD activity was established. The CD3\u2010induced increase in total inositol phosphates (InsP) in permeabilized cells was similar to the stimulated production of [<jats:sup>3<\/jats:sup>H] PA production in both CD45<jats:sup>+<\/jats:sup> and CD45<jats:sup>\u2212<\/jats:sup> cells. Dose\u2010response curves for InsP and PA production triggered by CD3 mAb were super\u2010imposable and the production of InsP and PA over a range of Ca<jats:sup>2+<\/jats:sup> concentrations was comparable. Differential labeling of phospholipids with <jats:sup>3<\/jats:sup>H\u2010labeled fatty acids revealed that CD3\u2010induced PA production reflected incorporation of label into the phosphatidylinositol pool. Our data suggest that in HPB\u2010ALL cells the production of DAG following CD3\u2010antigen cross\u2010linking can be fully accounted for by the selective coupling of the TCR to breakdown of phosphatidylinositol\u2010(4,5)\u2010bisphosphate as the result of phospholipase C\u03b3<jats:sub>1<\/jats:sub> activation. This event correlates with the activity of the CD45\u2010regulated TCR\u2010associated tyrosine kinase, p59<jats:sup><jats:italic>fyn<\/jats:italic><\/jats:sup>.<\/jats:p>","DOI":"10.1002\/eji.1830231138","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:30:29Z","timestamp":1172773829000},"page":"2980-2987","source":"Crossref","is-referenced-by-count":8,"title":["Selective coupling of the T cell antigen receptor to phosphoinositide\u2010derived diacylglycerol production in HPB\u2010ALL T cells correlates with CD45\u2010regulated p59<sup><i>fyn<\/i><\/sup> activity"],"prefix":"10.1002","volume":"23","author":[{"given":"Mark","family":"Biffen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Shiroo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis R.","family":"Alexander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.001035"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.12.12.5438"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220142"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05595.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220510"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90309-Z"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90598-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49749-4"},{"key":"e_1_2_1_10_2","first-page":"683","volume":"6","author":"Sefton B. 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