{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:26:38Z","timestamp":1770477998910,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":4224,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Taking advantage of the reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR), we have analyzed T cell receptor \u03b3\u2010chain mRNA of synovial fluid \u03b3\/\u03b4 T cells from patients with rheumatoid arthritis (RA) in comparison with those of peripheral blood mononuclear cells (PBMC) from RA patients and healthy individuals. The quantitative RT\u2010PCR method in conjunction with nucleotide sequencing revealed the frequent usage of the V\u03b33 gene segment in RA synovial fluid mononuclear cells (SFMC) (<jats:italic>p<\/jats:italic> &lt; 0.01) which in PBMC of healthy individuals occurred rarely. PBMC of most healthy individuals expressed the V\u03b39 gene predominantly (<jats:italic>p<\/jats:italic> &lt; 0.01) as expected. However, only half of RA patients showed elevated levels of the V\u03b39 gene expression in their PBMC. The \u03b3\u2010chain mRNA containing the V\u03b33 gene in RA SFMC showed no conserved junctional sequence (complementarity\u2010determining region 3). To investigate the nature of ligands recognized by the V\u03b33\u2010bearing T cells, we analyzed V\u03b3 gene usage of RA SFMC, RA PBMC, and normal PBMC stimulated with <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MT) or MT plus interleukin\u20102 since there is mounting evidence of high reactivity of RA SFMC to MT and mycobacterial heat\u2010shock protein 65. However, the V\u03b3 usage appeared to be mostly V\u03b39 in RA SFMC, RA PBMC and normal PBMC. Taken together these results suggest that an as yet unknown antigen(s) (other than MT) might select \u03b3\/\u03b4 T cells expressing the V\u03b33 gene in RA SFMC.<\/jats:p>","DOI":"10.1002\/eji.1830240517","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T21:51:36Z","timestamp":1172785896000},"page":"1122-1129","source":"Crossref","is-referenced-by-count":18,"title":["The biased V\u03b3 gene usage in the synovial fluid of patients with rheumatoid arthritis"],"prefix":"10.1002","volume":"24","author":[{"given":"Yasunori","family":"Kageyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Koide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigehito","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takato O.","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01857221"},{"key":"e_1_2_1_3_2","first-page":"22","volume":"49","author":"Duke O.","year":"1982","journal-title":"Clin. 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