{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T11:07:51Z","timestamp":1770721671497,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":4224,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The antigen\u2010presenting cell (APC) requirements for the <jats:italic>in vivo<\/jats:italic> induction of Th1\u2010and Th2\u2010type responses were investigated using a severe combined immunodeficiency (SCID)mouse chimera model. SCID mice adoptively transferred with either T cells [SCID(T)] or T + B cells [SCID(T + B)] and immunized with antigen in adjuvant were able to generate antigen\u2010specific T cells which could produce both interferon (IFN)\u2010\u03b3 and interleukin (IL)\u20104 upon <jats:italic>in vitro<\/jats:italic> restimulation. This suggests that B cell APC are not necessary for the priming of either IFN\u2010\u03b3\u2010 or IL\u20104\u2010producing T cells <jats:italic>in vivo.<\/jats:italic> The ability of different APC to activate Th2\u2010dependent effector mechanisms was also investigated. SCID(T) and SCID(T + B) mice were infected with the nematode parasite <jats:italic>Nippostrongylus brasiliensis<\/jats:italic> and analyzed for the development of IL\u20105\u2010dependent peripheral blood eosinophilia. Following infection both SCID(T) and SCID(T + B) mice generated similar numbers of peripheral blood eosilnophils, suggesting that similar amounts of IL\u20105 had been produced. Therefore, B cell APC are also not required for the <jats:italic>in vivo<\/jats:italic> activation of Th2 cells to lymphokine production. To establish more precisely which APC prime T cells to produce IFN\u2010\u03b3 and IL\u20104, normal mice were immunized by injection of syngeneic splenic dendritic cells which had been pulsed with antigen <jats:italic>in vitro.<\/jats:italic> T cells from these immunized mice were able to produce good IFN\u2010\u03b3 and IL\u20104 responses upon <jats:italic>in vitro<\/jats:italic> restimulation with specific antigen; therefore, dendritic cells appear to be sufficient APC for the <jats:italic>in vivo<\/jats:italic> priming of both IFN\u2010\u03b3\u2010 and IL\u20104\u2010producing T cells.<\/jats:p>","DOI":"10.1002\/eji.1830240521","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T21:51:36Z","timestamp":1172785896000},"page":"1148-1154","source":"Crossref","is-referenced-by-count":32,"title":["Interferon\u2010\u03b3\u2010 and interleukin\u20104\u2010producing T cells can be primed on dendritic cells <i>in vivo<\/i> and do not require the presence of B cells"],"prefix":"10.1002","volume":"24","author":[{"given":"Franca","family":"Ronchese","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Hausmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham Le","family":"Gros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115840"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.5.1753"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.1.383"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.2.631"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.1.15"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.1.239"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.5.1431"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3160115"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.001045"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.4049\/jimmunol.145.9.2803","volume":"145","author":"Chang T. 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