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As observed by skin test in virally infected mice challenged with viral peptides, the intrafootpad injection of tum<jats:sup>\u2212<\/jats:sup> peptides resulted in a dose\u2010dependent DTH that peaked at approximately 24 h. The response was mediated by CD8<jats:sup>+<\/jats:sup> cells and could be induced by previous vaccination of mice with live tumor cells, intrasplenic deposition of the eliciting peptide, or adoptive transfer with peptide\u2010pulsed syngeneic dendritic cells. These sensitization procedures resulted in an immunologically specific footpad reaction detectable for up to 2\u20136 months after priming. The evaluation by DTH in cancer patients of long\u2010lived CD8<jats:sup>+<\/jats:sup> anti\u2010tumor T cell responses following local challenge with tumor\u2010specific peptides may be of great interest in human immunotherapy trials involving immunization against identified tumor antigens.<\/jats:p>","DOI":"10.1002\/eji.1830240631","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T21:42:08Z","timestamp":1172785328000},"page":"1446-1452","source":"Crossref","is-referenced-by-count":25,"title":["Use of a skin test assay to determine tumor\u2010specific CD8<sup>+<\/sup> T cell reactivity"],"prefix":"10.1002","volume":"24","author":[{"given":"Paolo","family":"Puccetti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Bianchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria C.","family":"Fioretti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emira","family":"Ayroldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Uyttenhove","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"van Pel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Boon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Grohmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"3688","DOI":"10.4049\/jimmunol.138.11.3688","volume":"138","author":"Cher D. 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