{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:17:15Z","timestamp":1770704235765,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":4071,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>RJ 2.2.5 is a human B cell mutant derived from the Burkitt lymphoma Raji cell, which is defective in the AIR\u20101 locus function. This locus encodes a transcriptional trans\u2010activator required for the constitutive expression of major histocompatibility complex (MHC) class II genes. Here we show, by <jats:italic>in vivo<\/jats:italic> DNase I footprinting, that the AIR\u20101 locus defect correlates with changes in the DRA promoter occupancy. Interestingly, reexpression of human MHC class II genes in RJ 2.2.5 \u00d7 mouse spleen cell hybrids is associated with partial reversion of DRA promoter occupancy to the Raji pattern. DRA promoter occupancy in other class II\u2010negative B cell lines, derived from patients with bare lymphocyte syndrome, is drastically different from the one observed in RJ 2.2.5 and Raji cells. Moreover, the use of the DNase I as an <jats:italic>in vivo<\/jats:italic> footprinting agent reveals that the patients' cell lines do not display a completely \u201cbare promoter\u201d as previously reported using dimethyl sulfate as the footprinting agent. Thus, the use of DNase I allowed us, for the first time, to correlate the AIR\u20101 locus defect with class II promoter occupancy alterations and distinguish these alterations from the ones observed in phenotypically similar but genetically distinct MHC class II\u2010negative cells.<\/jats:p>","DOI":"10.1002\/eji.1830241023","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:59:55Z","timestamp":1172779195000},"page":"2415-2420","source":"Crossref","is-referenced-by-count":8,"title":["<i>In vivo<\/i> modification of major histocompatibility complex class II DRA promoter occupancy mediated by the AIR\u20101 trans\u2010activator"],"prefix":"10.1002","volume":"24","author":[{"given":"Gildas","family":"Rigaud","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiorenza","family":"Paiola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto S.","family":"Accolla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90068-0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.000305"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.3.1053"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.4049\/jimmunol.139.7.2489","volume":"139","author":"Calman A. 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