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The absence of perforin\u2010mediated cytotoxicity resulted in delayed clearance of Listeria from the spleen but not the liver after primary infection, overall susceptibility to Listeria however was not increased. Protection against a secondary infection was drastically impaired in perforin\u2010deficient mice. Adoptive transfer of immune spleen cells to recipients revealed that anti\u2010Listeria protection by CD8<jats:sup>+<\/jats:sup> T cells from perforin\u2010deficient versus normal mice was about 10\u2010fold reduced in livers and about 100\u2010fold reduced in the spleen of recipients. CD4<jats:sup>+<\/jats:sup> T cells from immune control and perforin\u2010deficient mice conferred comparable protection. These results indicate that the protective effect of CD8<jats:sup>+<\/jats:sup> T cells against an intracellular bacterium mainly evident in secondary infection is mediated by a perforin\u2010dependent pathway, presumably cytotoxicity, and less by other direct or indirect effector mechanisms.<\/jats:p>","DOI":"10.1002\/eji.1830241223","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:52:12Z","timestamp":1172778732000},"page":"3068-3072","source":"Crossref","is-referenced-by-count":164,"title":["CD8<sup>+<\/sup> T cell\u2010mediated protection against an intracellular bacterium by perforin\u2010dependent cytotoxicity"],"prefix":"10.1002","volume":"24","author":[{"given":"David","family":"K\u00e4gi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Ledermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"B\u00fcrki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Hengartner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf M.","family":"Zinkernagel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.259"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"2892","DOI":"10.1128\/iai.59.9.2892-2900.1991","volume":"59","author":"Dunn P. 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