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We have used this plasmid to transfect the mouse EL4 T cell line, which can, under certain conditions, produce IL\u20105 transcripts. Phorbol 12\u2010myristate 13\u2010acetate, A23187 and N<jats:sup>6<\/jats:sup>,2\u2032\u2010O\u2010dibutyryl\u2010adenosine 3\u2032:5\u2032\u2010cyclic monophosphate co\u2010stimulation of EL4 cells transfected with the human IL\u20105\/luciferase reporter gene construct resulted in maximal induction of the luciferase gene. Deletion analysis of the IL\u20105 promoter revealed the presence of negative regulatory elements between nucleotides \u2212404 and \u2212312 and two regions, located between nucleotides \u2212312 and \u2212227 and between nucleotides \u221280 and \u221235, that are involved in the positive regulation of the IL\u20105 promoter. Using electrophoretic mobility shift assays, we show that the positive element located between nucleotides \u2212312 and \u2212227 involves the binding of factors antigenically related to Oct1, Oct2A and Oct2B, to a perfect octamer motif located at position \u2212244\/\u2212237. Introduction of three point mutations in the octamer motif of the IL\u20105\/luciferase reporter gene plasmid, which results in the loss of competition for the factors binding to the IL\u20105 promoter sequence, reduced the production of luciferase from stimulated, transfected EL4 cells, by 90%. Octamer factors can also bind within the second positive regulatory region.<\/jats:p>","DOI":"10.1002\/eji.1830250544","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:07:04Z","timestamp":1172797624000},"page":"1431-1435","source":"Crossref","is-referenced-by-count":37,"title":["Characterization of the human interleukin\u20105 gene promoter: involvement of octamer binding sites in the gene promoter activity"],"prefix":"10.1002","volume":"25","author":[{"given":"Val\u00e9rie","family":"Gruart\u2010Gouilleux","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Engels","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.219"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1084\/jem.167.1.43","volume":"167","author":"Yamaguchi Y.","year":"1988","journal-title":"J. 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