{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:06:56Z","timestamp":1783098416445,"version":"3.54.6"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":3853,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have purified HLA\u2010DR from the spleen of a patient with rheumatoid arthritis. The patient had Felty's syndrome and was heterozygous for the DR4Dw4 antigen. We have isolated endogenous peptides from purified HLA\u2010DR molecules. The peptides were purified by reverse phase HPLC and the major peaks were subjected to N\u2010terminal sequencing. The peptides were derived from a variety of proteins: human serum albumin, human erythroid protein 4.1, 60S ribosomal proteins L31 and L35, VCAM\u20101, human immunoglobulin \u03bb chain and cathepsin\u2010S. A peptide corresponding to the sequence of human serum albumin (HSA) residues 106\u2013120 was synthesized and shown to bind to HLA\u2010DR4Dw4 (IC<jats:sub>50<\/jats:sub> = 1.41 \u03bcM). We have confirmed and refined current ideas about the structural motif for the binding of peptides to HLA\u2010DR and HLA\u2010DR4Dw4.<\/jats:p>","DOI":"10.1002\/eji.1830250553","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:07:04Z","timestamp":1172797624000},"page":"1473-1476","source":"Crossref","is-referenced-by-count":11,"title":["Purification and characterization of endogenous peptides extracted from HLA\u2010DR isolated from the spleen of a patient with rheumatoid arthritis"],"prefix":"10.1002","volume":"25","author":[{"given":"Robert D.","family":"Gordon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice A.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Rayner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W. A.","family":"Luke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy L.","family":"Crowther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Wordsworth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giles","family":"Hassall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue A.","family":"Hinchliffe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Culbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Davison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"305","volume":"11","author":"Gorga J. C.","year":"1992","journal-title":"Crit. Rev. Immunol."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.003423"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90184-E"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.5.1849"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/358764a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.1.27"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1319610"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.4049\/jimmunol.150.2.499","volume":"150","author":"Newcomb J. R.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/353622a0"},{"key":"e_1_2_1_11_2","first-page":"585","volume":"51","author":"Wordsworth P.","year":"1992","journal-title":"Am. J. Hum. Genet."},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"16087","DOI":"10.1016\/S0021-9258(18)47699-5","volume":"62","author":"Gorga","year":"1987","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.4049\/jimmunol.149.6.1994","volume":"149","author":"Schierle A.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_14_2","first-page":"295","volume":"8","author":"Akrigg D. A.","year":"1992","journal-title":"Computer Applications in the Biosciences"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.10.4456"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.4049\/jimmunol.146.7.2331","volume":"146","author":"Kreiger J. I.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"2890","DOI":"10.4049\/jimmunol.152.6.2890","volume":"152","author":"Hill M. C.","year":"1994","journal-title":"J. Immunol."},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/368215a0"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830250553","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830250553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T20:10:37Z","timestamp":1695759037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830250553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,5]]}},"alternative-id":["10.1002\/eji.1830250553"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830250553","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5]]}}}