{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T09:00:46Z","timestamp":1770714046546,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":3822,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have analyzed the expression of V<jats:sub>H<\/jats:sub> gene families in IgM, IgD and IgG of peripheral blood B cells from a group of HIV\u2010infected patients. CD19<jats:sup>+<\/jats:sup>CD20<jats:sup>+<\/jats:sup> cells were purified and anchored reverse transcriptase\u2010polymerase chain reaction products were hybridized with V<jats:sub>H<\/jats:sub> gene family probes. IgM, IgD and IgG that expressed a V<jats:sub>H<\/jats:sub>3 gene family segment, were decreased in patients with low CD4 counts and to a greater extend in patients with AIDS symptoms (up to 85% for IgG) compared to adult healthy donors. This was correlated with elevated levels of IgM and IgG encoded by a V<jats:sub>H<\/jats:sub>1 gene family segment (around 60% for IgG). These results confirm and extend previous work that has detected the V<jats:sub>H<\/jats:sub>3 gene family under\u2010representation in HIV infection. Here, we show that, <jats:italic>in vivo<\/jats:italic>, this phenomenon actually affects the different B cell populations of the peripheral blood: IgM<jats:sup>+<\/jats:sup> or IgG<jats:sup>+<\/jats:sup> B cells and also IgM<jats:sup>+<\/jats:sup>IgD<jats:sup>+<\/jats:sup> naive B cells. In the course of HIV infection, this results in their gradual depletion. Data presented here strengthen the hypothesis that a B cell superantigen exists in HIV infection. These pronounced variations of the normally most\u2010expressed V<jats:sub>H<\/jats:sub> gene family may be related to B cell abnormalities detected in HIV\u2010infected patients.<\/jats:p>","DOI":"10.1002\/eji.1830250608","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T00:48:40Z","timestamp":1172796520000},"page":"1524-1528","source":"Crossref","is-referenced-by-count":27,"title":["Selective variations <i>in vivo<\/i> of V<sub>H<\/sub>3 and V<sub>H<\/sub>1 gene family expression in peripheral B cell IgM, IgD and IgG during HIV infection"],"prefix":"10.1002","volume":"25","author":[{"given":"Denis","family":"David","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Demaison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynda","family":"Bani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moncef","family":"Zouali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Th\u00e8ze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0193-88"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0694-162"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/1.4.362"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(90)90161-R"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.4049\/jimmunol.146.8.2855","volume":"146","author":"Zouali M.","year":"1991","journal-title":"J. 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