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Thereafter, the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells decreased to a normal level by day 14. The TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells showed an activated T cell phenotype (L\u2010selectin\u2010CD44<jats:sup>+<\/jats:sup>) and expressed CD45\/B220 and interleukin\u20102 receptor \u03b2, but did not express heat stable antigen, which is expressed by the immature CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> thymocytes. Furthermore, 20\u201330% of the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells expressed the NK1.1 natural killer cell marker. Analyses of the TCR V region repertoire of the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells induced by <jats:italic>L. monocytogenes<\/jats:italic> infection showed that more than 80% of the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells expressed TCR V\u03b28 detected by anti\u2010TCR V\u03b28.1 and 8.2 mAb, and a reverse transcription\u2010polymerase chain reaction analysis of V\u03b114 relative to V\u03b111 expression revealed that the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells expressed a higher level of V\u03b114, which was reported to be preferentially expressed by TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> thymocytes rather than conventional CD4<jats:sup>+<\/jats:sup> T cells. The TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells showed a proliferative response to anti\u2010TCR\u03b1\u03b2 mAb stimulation. In contrast, they showed no response to stimulation with either <jats:italic>Listeria<\/jats:italic> antigen or 65\u2010kDa heat shock protein of <jats:italic>Mycobacterium bovis<\/jats:italic>, which do stimulate the <jats:italic>Listeria<\/jats:italic>\u2010specific TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells and the <jats:italic>Listeria<\/jats:italic>\u2010induced TCR \u03b3\u03b4<jats:sup>+<\/jats:sup> T cells, respectively. These results suggest that the TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> T cells may recognize a restricted set of self antigens induced by <jats:italic>L. monocytogenes<\/jats:italic> infection, and that they contribute to host protection at an early stage of the infection.<\/jats:p>","DOI":"10.1002\/eji.1830250728","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T00:47:23Z","timestamp":1172796443000},"page":"1985-1991","source":"Crossref","is-referenced-by-count":11,"title":["Early appearance of T cell receptor \u03b1\u03b2<sup>+<\/sup> CD4<sup>\u2212<\/sup> CD8<sup>\u2212<\/sup> T cells with a skewed variable region repertoire after infection with <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"25","author":[{"given":"Goro","family":"Matsuzaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Yan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Kadena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Hiromatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kikuo","family":"Nomoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1991.tb01592.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.4049\/jimmunol.146.4.1113","volume":"146","author":"Levitsky H. 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