{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:02:09Z","timestamp":1791223329541,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3767,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mice bearing a transgene coding for a soluble tumor necrosis factor receptor type 1 (TNFR1)\u2010FeIgG3 fusion protein and placed under the control of the alpha\u20101\u2010antitrypsin gene promoter were generated. Depending on the mouse line, blood levels of the protein ranged from 25 ng\/ml to over 100 \u03bcg\/ml; this level of expression was most often transmitted to the transgene\u2010bearing progeny as a relatively stable feature. High\u2010expressor mice were completely resistant to lipopolysaccharide\u2010induced shock and lethality, including after <jats:sc>D<\/jats:sc>\u2010galactosamine sensitization, and mice expressing about 1 \u03bcg of the fusion protein\/ml were partially (60%) protected. In contrast, mice expressing less than 0.1 \u03bcg of the protein\/ml were more sensitive than controls with respect to incidence and time of death, even though the biological activity of serum tumor necrosis factor (TNF) was partially neutralized. High\u2010expressor mice of the adequate genetic background were markedly, although not completely, protected from death by cerebral malaria after injection with <jats:italic>Plasmodium berghei<\/jats:italic>. They were highly susceptible to <jats:italic>Listeria monocytogenes<\/jats:italic>, dying from bacterial dissemination after sublethal infection, and to <jats:italic>Leishmania major<\/jats:italic>, displaying severe, non\u2010healing lesions after local infection. Under the same conditions, mice expressing about 1 \u03bcg protein\/ml were only partially sensitive to these last agents, compared to nontransgenic littermate mice which were fully resistant. These transgenic mice represent a model of permanent, complete or partial, impairment of TNF use, which compares favorably, for ease of breeding and for the range of effects, to mice bearing gene disruptions.<\/jats:p>","DOI":"10.1002\/eji.1830250841","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:17:47Z","timestamp":1172798267000},"page":"2401-2407","source":"Crossref","is-referenced-by-count":96,"title":["Transgenic mice expressing high levels of soluble TNF\u2010R1 fusion protein are protected from lethal septic shock and cerebral malaria, and are highly sensitive to <i>Listeria monocytogenes<\/i> and <i>Leishmania major<\/i> infections"],"prefix":"10.1002","volume":"25","author":[{"given":"Ir\u00e9ne","family":"Garcia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimi","family":"Araki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatake","family":"Araki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Lucas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges E.","family":"Grau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e9ve","family":"Milon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmine","family":"Belkaid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Montixi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Lesslauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Vassalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.002211"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.20.9292"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(92)90116-O"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02918612"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830211134"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.6.2173"},{"key":"e_1_2_1_8_2","first-page":"455","volume-title":"Bacterial endotoxin: recognition and effector mechanisms. Endotoxin Research, Series","author":"Loetscher H.","year":"1993"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90134-C"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/364798a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/372560a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00316a003"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90815-V"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"18 324","DOI":"10.1016\/S0021-9258(18)55273-X","volume":"266","author":"Loetscher H.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(84)90147-9"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3306918"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.5.1259"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.3.657"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"2894","DOI":"10.4049\/jimmunol.143.9.2894","volume":"143","author":"Havell E. A.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1128\/iai.56.10.2563-2569.1988","volume":"56","author":"Nakane A.","year":"1989","journal-title":"Infect. Immun."},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1989.tb00554.x"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.6.2097"},{"key":"e_1_2_1_23_2","first-page":"570","volume":"69","author":"Liew F. Y.","year":"1990","journal-title":"Immunology"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0769-2609(87)90151-7"},{"key":"e_1_2_1_25_2","first-page":"6108","volume":"49","author":"Sepulveda A. R.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3264419"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"11 974","DOI":"10.1016\/S0021-9258(18)80161-2","volume":"264","author":"Seckinger P.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_28_2","first-page":"20","volume":"76","author":"Girardin E.","year":"1992","journal-title":"Immunology"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80161-8"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.4049\/jimmunol.151.3.1548","volume":"151","author":"Mohler K. M.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.2.323"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830250841","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830250841","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:49:03Z","timestamp":1698367743000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830250841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["10.1002\/eji.1830250841"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830250841","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]}}}