{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:17:08Z","timestamp":1790720228755,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3736,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously demonstrated that at birth most productive (P) V<jats:sub>H<\/jats:sub>12 rearrangements in B10.H\u20102<jats:sup>a<\/jats:sup>H\u20104<jats:sup>b<\/jats:sup>p\/Wts (2<jats:sup>a<\/jats:sup>4<jats:sup>b<\/jats:sup>) mice encode a ten\u2010amino acid CDR3, and that a significant fraction of the expected repertoire is absent. We have now examined the adult V<jats:sub>H<\/jats:sub>12 CDR3 repertoire involving all four J<jats:sub>H<\/jats:sub> gene segments in both peritoneum and spleen. Of the 74 P V<jats:sub>H<\/jats:sub>12 rearrangements from these tissues 67 encode a CDR3 of ten amino acids and include a Gly in the fourth position (designated 10\/G4). Most of these rearrangements appear to derive from phosphatidylcholine (PtC)\u2010specific B cells, which also have a 10\/G4 V<jats:sub>H<\/jats:sub>CDR3, since few 10\/G4 P rearrangements were present in spleen cells depleted of PtC\u2010specific B cells. Thus, the V<jats:sub>H<\/jats:sub>12 B cell repertoire in adult mice is largely restricted to the use of a single CDR3 motif and to a single antigen specificity. This bias results from two selection events: (1) selective exclusion of most V<jats:sub>H<\/jats:sub>12 B cells from the peripheral repertoire, and (2) clonal expansion in the periphery of V<jats:sub>H<\/jats:sub>12 B cells that have a 10\/G4 V<jats:sub>H<\/jats:sub>CDR3 and bind PtC. Analysis of V<jats:sub>H<\/jats:sub>12\u2010J<jats:sub>H<\/jats:sub>1 rearrangements in viable motheaten (me<jats:sup>v<\/jats:sup>Jme<jats:sup>v<\/jats:sup>) mice, which have an abnormal B cell repertoire due to a defective phosphatase (Hcph) and have barely detectable numbers of PtC\u2010specific B cells, indicates that selective exclusion of V<jats:sub>H<\/jats:sub>12 B cells from the peripheral repertoire occurs normally, but that clonal expansion of 10\/G4 V<jats:sub>H<\/jats:sub>12 B cells is minimal. This is evidence that the selective exclusion of V<jats:sub>H<\/jats:sub>12 B cells from the peripheral repertoire and the clonal expansion of V<jats:sub>H<\/jats:sub>12 B cells with a 10\/G4 CDR3 are due to independent signaling events.<\/jats:p>","DOI":"10.1002\/eji.1830250916","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:17:30Z","timestamp":1172798250000},"page":"2511-2521","source":"Crossref","is-referenced-by-count":10,"title":["The majority of murine V<sub>H<\/sub>12\u2010expressing B cells are excluded from the peripheral repertoire in adults"],"prefix":"10.1002","volume":"25","author":[{"given":"Jian","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne K.","family":"McCray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.8039"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/342385a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.6.1357"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/5.11.1365"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.3.843"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/2.1.15"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.4049\/jimmunol.147.4.1406","volume":"147","author":"Decker D. 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