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To generate these \u03b1 P14 mice, mice transgenic for the P14 TCR \u03b1 chain were backcrossed to TCR \u03b1\u2010deficient mice. Thymi from \u03b1 P14 mice exhibited a marked decrease of mature CD4<jats:sup>+<\/jats:sup>8<jats:sup>\u2212<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>4<jats:sup>\u2212<\/jats:sup> single\u2010positive thymocytes comparable to thymi from TCR \u03b1\u2010deficient mice. Correspondingly, the number of peripheral T cells was reduced in the CD4 (tenfold) and in the CD8 (twofold) subsets when compared to normal mice. T cells from \u03b1 P14 mice generated a primary anti\u2010LCMV CTL response when stimulated <jats:italic>in vitro<\/jats:italic> with LCMV in contrast to normal mice which require priming <jats:italic>in vivo<\/jats:italic>; elimination of LCMV <jats:italic>in vivo<\/jats:italic> was, however, not improved. Flow cytometric analysis of T cells with V\u03b2\u2010specific antibodies showed a diverse endogenous TCR V\u03b2 repertoire. Functional analysis of the T cell repertoire, however, revealed a strongly reduced (30\u2010fold) allogeneic and the absence of a vesicular stomatitis virus\u2010specific CTL response and an impaired ability to provide T cell help for antibody isotype switching. Thus, T cell selection in the thymus was impaired and the T cell repertoire was limited in mice expressing only one type of TCR \u03b1 chain.<\/jats:p>","DOI":"10.1002\/eji.1830250937","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:17:30Z","timestamp":1172798250000},"page":"2650-2655","source":"Crossref","is-referenced-by-count":23,"title":["T cell development and repertoire of mice expressing a single T cell receptor \u03b1 chain"],"prefix":"10.1002","volume":"25","author":[{"given":"Daniel","family":"Br\u00e4ndle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Brduscha\u2010Riem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian C.","family":"Hayday","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Owen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Hengartner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80070-U"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973703"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.002531"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(92)90044-8"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/314103a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/315232a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90008-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8211163"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90425-4"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2970670"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/341742a0"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200227"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/334156a0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90453-J"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.20.9529"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1807"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/340559a0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03431.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.18.7135"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.4049\/jimmunol.146.9.2960","volume":"146","author":"Perkins D. 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