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CTL recognition of such determinants is always sequencedependent due to co\u2010recognition of TNP as well as amino acid side chains of the carrier peptide. By the use of glycine\u2010based designer peptides for primary induction of CTL <jats:italic>in vitro<\/jats:italic>, we have identified two sub\u2010epitopes on individual position 7\u2010haptenated peptides that form two TcR contact points and which can be independently recognized by cloned CTL. One of these sub\u2010epitopes is represented by the hapten itself, the other by the amino acids tyrosine and lysine in positions 3 and 4 of the carrier peptide, respectively. Immunization with such TNP\u2010modified peptides frequently results in the specific induction of CTL also reacting with the unmodified carrier peptides. DNA sequence analyses of the TcR revealed an extraordinary similarity of several independent TcR of CTL from individual mice and induced with different TNP\u2010peptides. These receptor similarities clearly correlate with structural elements common to the immunizing peptides and suggest their origin from positive thymic selection of TcR on K<jats:sup>b<\/jats:sup>\u2010associated self\u2010peptides bearing Tyr in position 3. Our data provide additional information concerning the topology of TcR binding to peptide\/MHC complexes with, but also without, TNP. They also indicate a mechanism which might explain the potential of chemicals or drugs to induce autoimmune phenomena.<\/jats:p>","DOI":"10.1002\/eji.1830251012","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:39:23Z","timestamp":1172792363000},"page":"2788-2796","source":"Crossref","is-referenced-by-count":17,"title":["Carrier\u2010reactive hapten\u2010specific cytotoxic T lymphocyte clones originate from a highly preselected T cell repertoire: implications for chemical\u2010induced self\u2010reactivity"],"prefix":"10.1002","volume":"25","author":[{"given":"Stefan","family":"Martin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga","family":"Ruh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Hebbelmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Pflugfelder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"R\u00fcde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Ulrich","family":"Weltzien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"629","volume-title":"Fundamental Immunology","author":"Germain R. 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