{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:14:40Z","timestamp":1770718480919,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3706,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>B cells that are destined to secrete IgE express a membrane\u2010bound form of IgE (mIgE) on their cell surface. Thus, elimination of such mIgE\u2010positive cells should result in the suppression of IgE production, thereby alleviating the symptoms of IgE\u2010mediated allergy. In this study, we examined, in a model system, whether IgE\u2010specific effector T cells can be used specifically to eradicate IgE\u2010producing B cells. To this end, we endowed T cells with anti\u2010IgE specificity using chimeric T cell receptors (cTCR) containing the variable region domain (Fv) of the 84.1c non\u2010anaphylactic anti\u2010mouse IgE monoclonal antibody (mAb). Two configurations of chimeric receptor were used: in the first, we combined the heavy and light variable region chains of 84.1c with the constant (C) regions of the TCR \u03b1 and \u03b2 chains. The second construct consisted of a chimeric single\u2010chain receptor (scFvR), composed of a single\u2010chain Fv region of the 84.1c antibody and the C\u03b2 domain of the TCR. Following transfection of the cTCR or the scFvR genes into the murine MD.45 cytotoxic T cell hybridoma or the Jurkat human T cell line, functional expression of IgE\u2010specific chimeric receptors was detected on the cell surface. The transfected cells secreted interleukin\u20102 upon stimulation with immobilized IgE or fixed IgE\u2010producing hybridoma cells. Moreover, cytotoxic T cell hybridomas expressing the chimeric receptor genes specifically eliminated IgE\u2010secreting B cells <jats:italic>in vitro<\/jats:italic>, resulting in isotype\u2010specific suppression of IgE production.<\/jats:p>","DOI":"10.1002\/eji.1830251041","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:39:23Z","timestamp":1172792363000},"page":"2985-2991","source":"Crossref","is-referenced-by-count":15,"title":["Specific elimination of IgE production using T cell lines expressing chimeric T cell receptor genes"],"prefix":"10.1002","volume":"25","author":[{"given":"Joseph","family":"Lustgarten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zelig","family":"Eshhar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.4049\/jimmunol.136.12.4565","volume":"136","author":"Robertson D.","year":"1986","journal-title":"J. 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