{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T07:39:55Z","timestamp":1779262795423,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3706,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The CD95 (APO\u20101\/Fas) ligand (CD95L) mediates apoptosis in sensitive target cells, Ca<jats:sup>2+<\/jats:sup>\u2010independent cytotoxicity of cells from perforin knock\u2010out mice, and peripheral deletion of activated T cells through engagement of its cognate receptor CD95. Double\u2010positive thymocytes show a high constitutive expression of CD95. Therefore, we used a model system and investigated whether negative selection through apoptosis might involve CD95\/CD95L. We analyzed whether CD95L may induce antigen\u2010specific deletion of double\u2010positive thymocytes from mice transgenic for a lymphocytic choriomeningitis virus (LCMV)\/H2<jats:sup>b<\/jats:sup>\u2010specific T cell receptor (TCR). These cells are deleted <jats:italic>in vitro<\/jats:italic> upon addition of the LCMV\u2010peptide 33\u201341 in a major histocompatibility complex\u2010class I\u2010restricted fashion. Deletion was not blocked by soluble mouse and human CD95\u2010Fc receptor decoys. CD95\u2010Fc receptor decoys, however, were effective in blocking apoptosis induced by mouse CD95L\u2010transfected L929 cells in sensitive CD95<jats:sup>+<\/jats:sup> target cells and in thymocytes. These results suggest that TCR\u2010induced deletion of immature thymocytes <jats:italic>in vitro<\/jats:italic> is independent of CD95L. Thus, our data argue against a role of CD95L in negative selection of MHC\u2010class I\u2010restricted autoreactive thymocytes.<\/jats:p>","DOI":"10.1002\/eji.1830251043","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:39:23Z","timestamp":1172792363000},"page":"2996-2999","source":"Crossref","is-referenced-by-count":13,"title":["Clonal deletion of major histocompatibility complex class I\u2010restricted CD4<sup>+<\/sup>CD8<sup>+<\/sup> thymocytes <i>in vitro<\/i> is independent of the CD95 (APO\u20101\/Fas) ligand"],"prefix":"10.1002","volume":"25","author":[{"given":"Klaus\u2010Peter","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara M.","family":"Mariani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Matiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Kyewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter H.","family":"Krammer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1994.tb00889.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7533326"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2787530"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.5.1747"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90326-L"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7518614"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/370650a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/373438a0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/373441a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/373444a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.12.1849"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60620-3"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240339"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.1.73"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830241231"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.485"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"4395","DOI":"10.4049\/jimmunol.154.9.4395","volume":"154","author":"Nishimura Y.","year":"1995","journal-title":"J. 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