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While the CD11a integrin subunit is expressed on all leukocytes, CD11c is almost exclusively expressed on cells of the myeloid lineage and on activated B lymphocytes. Its expression is regulated during cell activation and differentiation by transcriptional mechanisms. We have previously demonstrated that the proximal region of the CD11c promoter directs tissue\u2010restricted and developmentally\u2010regulated expression of reporter genes. Structural studies by electrophoretic mobility shift assays have demonstrated the presence of two Sp1\u2010binding sites at \u221270 (Sp1\u201370) and\u2212120 (Sp1\u2013120) which mediate the Sp1 transactivation of the CD11c promoter in Sp1\u2010defective SL2 cells, and which are involved in cell lineage\u2010specific DNA\u2010protein interactions, as demonstrated by footprinting <jats:italic>in vivo<\/jats:italic>. More importantly, mutation of either Sp1 site inhibited the activity of the CD11c promoter both in myeloid U937 cells and the CD11c\u2010expressing B lymphoblastoid JY cell line, while the opposite effect was observed in the CD11c\u2010negative epithelial HeLa cell line, demonstrating the involvement of both Sp1\u2010binding sites in the basal and the tissue\u2010restricted expression of the CD11c integrin subunit gene. Interestingly, the analysis of the CD11a proximal promoter also revealed the existence of an Sp1\u2010binding site at\u221270, indicating a common role for these <jats:italic>cis<\/jats:italic>\u2010acting elements in the transcription of the leukocyte integrin \u03b1 subunit genes. The binding of Sp1 to the regulatory regions of the leukocyte integrin genes raises the possibility that the retinoblastoma susceptibility gene product is implicated in integrin expression through its functional interaction with Sp1, thus establishing a link between integrin\u2010dependent leukocyte adhesiveness and the state of cellular differentiation\/proliferation.<\/jats:p>","DOI":"10.1002\/eji.1830251243","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:36:53Z","timestamp":1172792213000},"page":"3496-3503","source":"Crossref","is-referenced-by-count":28,"title":["Identification of Sp1\u2010binding sites in the CD11c (p150,95\u03b1) and CD11a (LFA\u20101\u03b1) integrin subunit promoters and their involvement in the tissuespecific expression of CD11c"],"prefix":"10.1002","volume":"25","author":[{"given":"Cristina","family":"L\u00f3pez\u2010Rodr\u00edguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Min","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Tenen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel L.","family":"Corb\u00ed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60653-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.5.1779"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170915"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"648","DOI":"10.4049\/jimmunol.146.2.648","volume":"146","author":"Stacker S. 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