{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T15:58:50Z","timestamp":1770739130472,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3583,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously described the cloning of a cDNA encoding an interleukin\u201012 receptor (IL\u201012R) subunit, designated \u03b2, that bound IL\u201012 with low affinity when expressed in COS cells. We now report that a pair of monoclonal antibodies (mAb), 2B10 and 2.4E6, directed against different epitopes on the IL\u201012R\u03b2 chain, when used in combination, strongly inhibited IL\u201012\u2010induced proliferation of activated T cells, IL\u201012\u2010induced secretion of interferon\u2010\u03b3 by resting peripheral blood mononuclear cells (PBMC), and IL\u201012\u2010mediated lymphokine\u2010activated killer cell activation. The mAb had no effect on lymphoblast proliferation induced by IL\u20102, \u22124, or \u22127. Thus, the IL\u201012R\u03b2 chain appears to be an essential component of the functional IL\u201012R on both T and natural killer (NK) cells. We previously observed that high affinity IL\u201012R were expressed on activated T and NK cells, but not B cells. Studies using flow cytometry and reverse transcription\u2010polymerase chain reaction analysis showed that IL\u201012R\u03b2 chain was expressed on several human T, NK, and (surprisingly) B cell lines, but not on non\u2010lymphohematopoietic cell lines. The Kit225\/K6 (T cell) and SKW6.4 (B cell) lines were found to express the greatest amounts of IL\u201012R\u03b2 chain (800\u20132500 sites\/cell); however, Kit225\/K6 but not SKW6.4 cells bound IL\u201012. Similar to SKW6.4 B cells, activated tonsillar B lymphocytes expressed IL\u201012R\u03b2 chain but, consistent with previous results, did not display detectable IL\u201012 binding. Likewise, up to 72% of resting PBMC from normal volunteer donors expressed IL\u201012R\u03b2, but did not bind measurable amounts of IL\u201012. These results indicate that expression of IL\u201012R\u03b2 is essential, but not sufficient, for expression of functional IL\u201012R. We speculate that expression of functional, high\u2010affinity IL\u201012R may require the presence of a second subunit that is more restricted in its expression than IL\u201012R\u03b2.<\/jats:p>","DOI":"10.1002\/eji.1830260212","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:55:43Z","timestamp":1172800543000},"page":"345-350","source":"Crossref","is-referenced-by-count":53,"title":["Biological function and distribution of human interleukin\u201012 receptor \u03b2 chain"],"prefix":"10.1002","volume":"26","author":[{"given":"Chang\u2010You","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev R.","family":"Warrier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy M.","family":"Carvajal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne O.","family":"Chua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa J.","family":"Minetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Chizzonite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia K. A.","family":"Mongini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin S.","family":"Stern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ueli","family":"Gubler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Presky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurice K.","family":"Gately","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.12.4008.bloodjournal84124008"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8097338"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.223"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.4049\/jimmunol.154.6.2578","volume":"154","author":"Dekruyff R. 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