{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:37:13Z","timestamp":1790480233836,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":3531,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The activation of T cells requires engagement of the T cell receptor\/CD3 complex and co\u2010stimulatory molecules, and results in the triggering of several signaling pathways which lead rapidly to the nuclear translocation of several transcription factors, such as nuclear factor (NF)\u2010\u03f0B and NF\u2010AT. A result of this activation process is the induction of a number of genes, including those encoding cytokines such as interleukin\u20102, tumor necrosis factor\u2010\u03b1, and interferon (IFN)\u2010\u03b3 which have important immunoregulatory effects. We report here that a DNA\u2010binding factor containing STAT1 also becomes activated in human peripheral blood T lymphocytes or Jurkat cells, although not until 1\u20132 h after stimulation. Activation is delayed a further 1\u20132 h when mononuclear cell cultures are stimulated by an antigen which requires processing. Appearance of the STAT1 factor is significantly reduced in the presence of cyclosporin A, and blocked by cycloheximide, indicating that its activation is dependent upon a protein(s) synthesized in response to initial signaling events. Neutralizing antiserum against IFN\u2010\u03b3, but not other cytokines tested, blocked activation of the factor almost completely, and IFN\u2010\u03b3 was found in the culture supernatants of stimulated cells at levels at which recombinant IFN\u2010\u03b3 could activate the factor in naive cells. Therefore, a STAT1 transcription factor is activated by IFN\u2010\u03b3 synthesized and released upon stimulation of T lymphocyte populations. While Jurkat cells both secret and respond to IFN\u2010\u03b3 in an autocrine loop, it seems likely that the responding cells may differ from those synthesizing this cytokine in the mononuclear cell cultures in the light of the recent report that Th1 cells lack the IFN\u2010\u03b3 receptor chain necessary for activation of STAT1 (Pernis, A., Gupta, S., Gollob, K. J., Garfein, E., Coffman, R. L., Schindler, C., and Rothman, P., <jats:italic>Science<\/jats:italic> 1995. <jats:italic>269<\/jats:italic>: 245).<\/jats:p>","DOI":"10.1002\/eji.1830260329","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:50:12Z","timestamp":1172800212000},"page":"704-709","source":"Crossref","is-referenced-by-count":35,"title":["Autocrine activation by interferon\u2010\u03b3 of STAT factors following T cell activation"],"prefix":"10.1002","volume":"26","author":[{"given":"John","family":"Girdlestone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Wing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.002225"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90152-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8197455"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90141-8"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90088-4"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90734-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06898.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90334-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830211111"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230216"},{"key":"e_1_2_1_12_2","volume-title":"Molecular Cloning.","author":"Maniatis T.","year":"1982"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.5.1475"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11568"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8397445"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90235-6"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7694370"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb08026.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90807-X"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180506"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.005145"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.299"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/smvy.1995.0022"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90007-8"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90056-6"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.7.3579"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.267.5195.240"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.16.3283"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80134-0"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618088"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06245.x"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90132-5"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456300"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830260329","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830260329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T16:49:44Z","timestamp":1698338984000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830260329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,4]]}},"alternative-id":["10.1002\/eji.1830260329"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830260329","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4]]}}}