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Dendritic cells process exogenous antigens conventionally for presentation on major histocompatibility complex (MHC) class II molecules. However, unconventional processing of exogenous antigens <jats:italic>in vitro<\/jats:italic> for presentation on MHC class I molecules is still an open question. In this study, we report that a cloned dendritic cell line (D2SC\/1) is able to present cell debris\u2010associated exogenous viral proteins to MHC class I\u2010restricted CTL <jats:italic>in vitro<\/jats:italic> The dendritic cell line was very efficient in processing recombinant lymphocytic choriomeningitis virus nucleoprotein (LCMV NP) and presenting the class I\u2010restricted epitope to CTL primed <jats:italic>in vivo<\/jats:italic>. Peritoneal macrophages could also process the recombinant LCMV NP for subsequent MHC class I presentation, but were less efficient compared to the dendritic cells. Furthermore, recombinant yeast\u2010derived virus\u2010like particles carrying the HIV\u20101 V3 loop (V3\u2010VLP), which are protenaceous and do not contain any lipid, were also found to be efficiently processed by the dendritic cell line for presentation of the class I\u2010restricted epitope. These results clearly indicate that viral proteins, in particulate form or associated with cell debris, are processed by dendritic cells for CTL induction.<\/jats:p>","DOI":"10.1002\/eji.1830261109","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:32:25Z","timestamp":1172799145000},"page":"2595-2600","source":"Crossref","is-referenced-by-count":113,"title":["Dendritic cells process exogenous viral proteins and virus\u2010like particles for class I presentation to CD8<sup>+<\/sup> cytotoxic T lymphocytes"],"prefix":"10.1002","volume":"26","author":[{"given":"Martin F.","family":"Bachmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfre D. 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