{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T11:07:00Z","timestamp":1770721620501,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":3273,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neonatally thymectomized (NTx) mice fall victim to such autoimmune diseases as gastritis and pancreatitis with aging. Self\u2010reactive T cell clones are known to be consistently generated through TCR intermediate (<jats:italic>i.e<\/jats:italic>. TCR<jats:sup>int<\/jats:sup>) cell differentiation in normal mice (<jats:italic>i.e<\/jats:italic>. via the extrathymic pathways and an alternative intrathymic pathway). It was investigated whether the generation of such clones in NTx mice follows this rule or whether they are generated by default via mainstream T cell differentiation in the thymus. The majority of T cells generated in NTx mice were TCR<jats:sup>int<\/jats:sup> cells in all organs tested. In contrast to athymic mice, which carry only TCR<jats:sup>int<\/jats:sup> cells with aging, a leaky appearance of high TCR (<jats:italic>i.e<\/jats:italic>. TCR<jats:sup>hi<\/jats:sup>) cells emerged in the lymph nodes and other organs of NTx mice. Self\u2010reactive clones estimated by anti\u2010V\u03b2 monoclonal antibodies in conjunction with the Mls system were confined to TCR<jats:sup>int<\/jats:sup> cells in all tested organs, including a target organ, the stomach, in NTx mice. A leaky population of TCR<jats:sup>hi<\/jats:sup> cells did not contain a significant number of self\u2010reactive clones. Moreover, such self\u2010reactive clones among TCR<jats:sup>int<\/jats:sup> cells in NTx mice with autoimmune disease were shown to be nonanergic in the proliferation assay. The present results suggest that the generation of self\u2010reactive clones is totally due to TCR<jats:sup>int<\/jats:sup> cell differentiation, although it is still undetermined whether the expanding TCR<jats:sup>int<\/jats:sup> cell population is generated via the extrathymic pathway or an alternative intrathymic pathway. It is shown here not to be due to a failure of the TCR<jats:sup>hi<\/jats:sup> cell\u2010differentiation pathway in NTx mice.<\/jats:p>","DOI":"10.1002\/eji.1830261239","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:29:25Z","timestamp":1172798965000},"page":"3084-3091","source":"Crossref","is-referenced-by-count":21,"title":["Restricted appearance of self\u2010reactive clones into T cell receptor intermediate cells in neonatally thymectomized mice with autoimmune disease"],"prefix":"10.1002","volume":"26","author":[{"given":"Tetsuya","family":"Moroda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneo","family":"Iiai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Kawachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Kawamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyoshi","family":"Hatakeyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Abo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"123","volume":"60","author":"Taguchi O.","year":"1985","journal-title":"Clin. 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