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The mutant mice generated are unable to express the V<jats:sub>H<\/jats:sub>81X gene but appear to display a normal pattern of B cell differentiation as well as normal numbers of bone marrow and peripheral B cells from fetal life all through ontogeny. They mount normal immune responses to several different antigens tested. In contrast, the distribution of V<jats:sub>H<\/jats:sub> gene rearrangements in the V<jats:sub>H<\/jats:sub>7183 family is altered in homozygous mutant mice. Thus, the antibody repertoire of the targeted mice is modified, at least as far as the expression of V<jats:sub>H<\/jats:sub>7183 genes is concerned.<\/jats:p>","DOI":"10.1002\/eji.1830270144","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T03:31:13Z","timestamp":1172806273000},"page":"307-314","source":"Crossref","is-referenced-by-count":4,"title":["Targeted disruption of the V<sub>H<\/sub> 81X gene: Influence on the B cell repertoire"],"prefix":"10.1002","volume":"27","author":[{"given":"Fran\u00e7ois","family":"Huetz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulla\u2010Carin","family":"Tornberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyne","family":"Malanch\u00e8re","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Roes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leif","family":"Carlsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Coutinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Holmberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Rajewsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3975629"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.2.589"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/1.4.342"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/311727a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.4049\/jimmunol.146.1.350","volume":"146","author":"Decker D. 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