{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:06:48Z","timestamp":1791198408407,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,5]],"date-time":"2005-12-05T00:00:00Z","timestamp":1133740800000},"content-version":"vor","delay-in-days":3201,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD95 ligation elicits apoptotic signals in many cell systems. This study analyzes the effect of anti\u2010CD95 mAb on human cells capable of spontaneous and highrate Ig secretion. Such cells have been induced <jats:italic>in vivo<\/jats:italic> and represent a highly mature B cell stage. Addition of the anti\u2010CD95 monoclonal antibody (mAb) CH11 to tonsil B cells inhibited 50\u201360% of their spontaneous Ig secretion. The effect was exerted early in the culture and could be reversed by a pre\u2010treatment with a neutralizing mAb. <jats:italic>N<\/jats:italic>\u2010acetyl\u2010D\u2010sphingosine (C2\u2010ceramide), although not a close analog, also reduced Ig secretion to a similar extent. The inclusion of a tetrapeptide inhibitor for certain interleukin\u20101\u03b2\u2010converting enzyme proteases prevented the inhibitory effect of CH11 mAb on tonsil B cells. B cells capable of spontaneous Ab secretion obtained from blood of recently\u2010immunized volunteers were also inhibited by CH11 mAb and C2\u2010ceramide. In contrast, bone marrow (BM) B cells capable of spontaneous Ig secretion were unaffected by these agents. This CD95 ligation\u2010mediated inhibition of tonsil and blood Ig\u2010secreting B cells could not be reversed by cytokines with demonstrated activity on these B cells. Human mature B cells induced <jats:italic>in vivo<\/jats:italic> are identifiable as CD38<jats:sup>hi<\/jats:sup> cells. Flow cytometric analysis revealed that a fraction of tonsil CD38<jats:sup>hi<\/jats:sup> cells expressed low levels of CD95. Moreover, about 20% of these cells exhibited basal apoptosis, as defined by annexin V binding. This phenomenon was markedly increased by CD95 ligation. On the other hand, BM CD38<jats:sup>hi<\/jats:sup> cells showed neither CD95 expression nor CD95\u2010induced annexin V binding. These data suggest that CD95 ligation might play a role in the control of human humoral responses by inducing apoptosis in susceptible mature B cells.<\/jats:p>","DOI":"10.1002\/eji.1830270319","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T04:32:52Z","timestamp":1172809972000},"page":"700-706","source":"Crossref","is-referenced-by-count":26,"title":["Regulatory role of CD95 ligation on human B cells induced <i>in vivo<\/i> capable of spontaneous and highrate Ig secretion"],"prefix":"10.1002","volume":"27","author":[{"given":"Francisco","family":"Medina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Segundo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Brieva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90614-5"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50076-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90326-L"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.10.3166"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/364806a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/376181a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.152.12.5624"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1265"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.77"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"5746","DOI":"10.4049\/jimmunol.154.11.5746","volume":"154","author":"Legresle C.","year":"1995","journal-title":"J. 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