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Some antigens, such as the lipid\u2010bound, native form of myelin basic protein (LB\u2010MBP) can also be presented by recycling of cell surface MHC class II molecules. The data reported here demonstrate that a preparation of highly purified, delipidated MBP (HP\u2010MBP) follows yet another presentation pathway. Similar to LB\u2010MBP, presentation of HP\u2010MBP to HLA\u2010DR1\u2010restricted T cells was independent of HLA\u2010DM, of newly synthesized proteins, and of invariant chain expression. However, in contrast to LB\u2010MBP, presentation of HP\u2010MBP was also independent of internalization of surface HLA\u2010DR molecules. The different requirements for the presentation of the two molecular forms of MBP were further confirmed by use of the protease inhibitor E64: presentation of LB\u2010MBP but not of HP\u2010MBP was inhibited after treatment of target cells with E64. Furthermore, intact HP\u2010MPB bound to isolated HLA\u2010DR molecules <jats:italic>in vitro<\/jats:italic> with an association rate that was considerably faster than that of short peptides. These results show that presentation of HP\u2010MBP is independent of intracellular processing and suggest that it may be presented to T cells by direct binding to surface HLA\u2010DR molecules.<\/jats:p>","DOI":"10.1002\/eji.1830270421","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T04:32:40Z","timestamp":1172809960000},"page":"941-951","source":"Crossref","is-referenced-by-count":38,"title":["HLA\u2010DR\u2010restricted presentation of purified myelin basic protein is independent of intracellular processing"],"prefix":"10.1002","volume":"27","author":[{"given":"Marco","family":"Vergelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Pinet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne B.","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Kalbus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Malnati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Riccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric O.","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/368551a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/368554a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90081-Z"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.6.1699"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"812","DOI":"10.4049\/jimmunol.145.3.812","volume":"145","author":"St. Pierre Y.","year":"1993","journal-title":"J. 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