{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:30:12Z","timestamp":1761597012111},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":3110,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>PE38 is a 38\u2010kDa derivative of the 66\u2010kDa <jats:italic>Pseudomonas<\/jats:italic> exotoxin (PE) in which the cell binding domain of PE (domain Ia, amino acids 1\u2013252) and a portion of domain Ib (amino acids 365\u2013380) are deleted. The immunotoxins LMB\u20101 and LMB\u20107 contain PE38 and kill cancer cells by exploiting the cytotoxic action of PE38. The major human B cell epitopes of PE38 were mapped by measuring the reactivity of 45 serum samples from patients treated with the PE38\u2010containing immunotoxins LMB\u20101 or LMB\u20107 to two panels of overlapping synthetic peptides representing the sequence of PE38. One panel of peptides is ten amino acids long and overlap by seven amino acids, and the second panel of peptides is twenty amino acids long and overlap by ten. Five major epitopes were identified: amino acids 274\u2013283, 470\u2013492, 531\u2013540, 555\u2013564, and the C\u2010terminal amino acids 596\u2013609. Two minor epitopes were identified as well: amino acids 501\u2013510 and 582\u2013589. These epitopes are predominantly located on the surface of the protein. The amino acids believed to be critical for binding are highly solvent\u2010accessible residues. The results of the human antibody response to peptides are compared to the pattern of reactivity previously identified with serum samples obtained from monkeys administered LMB\u20101 and LMB\u20107. The epitopes between monkey and human are almost identical, demonstrating similarity in the response of antibody repertoires between the two species and providing further support that these are the immunodominant epitopes. This information is critical for genetically engineering less immunogenic immunotoxins and provides a foundation for the development of a vaccine against pseudomonal infections which plague immunocompromised individuals and individuals with cystic fibrosis.<\/jats:p>","DOI":"10.1002\/eji.1830270624","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T05:00:39Z","timestamp":1172811639000},"page":"1459-1468","source":"Crossref","is-referenced-by-count":24,"title":["Identification of epitopes on a mutant form of <i>Pseudomonas<\/i> exotoxin using serum from humans treated with <i>Pseudomonas<\/i> exotoxin containing immunotoxins"],"prefix":"10.1002","volume":"27","author":[{"given":"Donna M.","family":"Roscoe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee H.","family":"Pai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira","family":"Pastan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90363-1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.6.2284"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.5.1320"},{"key":"e_1_2_1_5_2","first-page":"1","volume":"52","author":"Debinski W.","year":"1992","journal-title":"Cancer Res."},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"9470","DOI":"10.1016\/S0021-9258(19)76564-8","volume":"263","author":"Kondo T.","year":"1988","journal-title":"J. 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