{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:27:48Z","timestamp":1770719268360,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":3067,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vascular adhesion protein\u20101 (VAP\u20101) is an endothelial molecule which mediates lymphocyte binding to endothelium in peripheral lymph nodes and at certain sites of inflammation. The expression of VAP\u20101 <jats:italic>in vivo<\/jats:italic> is strongly up\u2010regulated in inflamed tissues, such as gut and skin. The purpose of this work was to examine the factors responsible for this induction of VAP\u20101. Since the expression of VAP\u20101 could not be induced in cultured endothelial cells with a large panel of mediators, we used an organ culture technique for the investigation of the regulation of VAP\u20101 expression in a more physiological micromilieu. Indeed, we found that the expression of endothelial VAP\u20101 could be up\u2010regulated in human tonsillar tissue with interleukin (IL)\u20101, IL\u20104, tumor necrosis factor (TNF\u2010\u03b1), interferon (IFN)\u2010\u03b3 and lipopolysaccharide, whereas histamine, thrombin, dibutyryl cAMP, N\u2010formyl\u2010Met\u2010Leu\u2010Phe (fMLP) and phorbol 12\u2010myristate 13\u2010acetate (PMA) had no effect. The induced VAP\u20101 protein was similar in molecular weight to the non\u2010induced VAP\u20101, suggesting that VAP\u20101 synthesized <jats:italic>de novo<\/jats:italic> carries appropriate carbohydrate moieties. In contrast to tonsil organ culture, similar inductions performed with human appendix showed no up\u2010regulation of VAP\u20101 expression, indicating that the regulation of VAP\u20101 expression exhibits organ\u2010selective characteristics. Furthermore, in these tissues the smooth muscle cells, which constitutively express VAP\u20101, could not be stimulated to alter their level of expression of this molecule. In conclusion, the expression of VAP\u20101 can be markedly up\u2010regulated with several mediators in tonsil but not in appendix organ culture, whereas cultured endothelial cells cannot be induced to express VAP\u20101. These results indicate that the expression of VAP\u20101 is regulated in a tissue\u2010 and cell type\u2010selective manner, and a correct micromilieu is required for the up\u2010regulation to occur.<\/jats:p>","DOI":"10.1002\/eji.1830270730","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T05:21:40Z","timestamp":1172812900000},"page":"1794-1800","source":"Crossref","is-referenced-by-count":31,"title":["Organ\u2010selective regulation of vascular adhesion protein\u20101 expression in man"],"prefix":"10.1002","volume":"27","author":[{"given":"Anna\u2010Maija","family":"Arvilommi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marko","family":"Salmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirpa","family":"Jalkanen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90337-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(96)04569-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.137.6.1893"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.137.1.245"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.145.3.819"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"2558","DOI":"10.4049\/jimmunol.144.7.2558","volume":"144","author":"Wellicome S. 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