{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T03:49:14Z","timestamp":1770695354397,"version":"3.49.0"},"reference-count":86,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":3013,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD43 (leukosialin), a sialylated glycoprotein expressed on the surface of most hematopoietic cells, has been implicated in cell adhesion and signaling. However, its precise physiological function remains unclear. We used mouse CD43 (mCD43)\u2010immunoglobulin enhancer\u2010transgenic (TG) mice to study the role of mCD43 <jats:italic>in vivo.<\/jats:italic> Previous work revealed that mCD43 expression on mature B cells in these mice resulted in immunodeficiency to T\u2010dependent (TD) antigens (Ag), possibly by impairing B\u2010T cell interactions. In the present study we have immunized the TG mice with the T\u2010independent (TI) Ag fluorescein\u2010(Fl) lipopolysaccharide (LPS) (TI type 1 Ag) and Fl\u2010Ficoll (TI type 2 Ag). Surprisingly, the mCD43\u2010Ig enhancer expressing mice were impaired in their ability to mount humoral responses to both Fl\u2010LPS and Fl\u2010Ficoll, and had decreased numbers of cells responding to Ag <jats:italic>in vivo.<\/jats:italic> Flow cytometric analysis was performed on peritoneal B\u20101 cells, a population which often plays a major role in humoral responses to TI Ag such as bacterial Ag. This analysis revealed similar B220, IgM and CD5 expression patterns for the TG and nontransgenic (NTG) B\u20101 cells. In addition, purified peritoneal B\u20101 cells from TG and NTG mice were able to respond to LPS. Stimulation of splenic B cells <jats:italic>in vitro<\/jats:italic> with Fl\u2010LPS and Fl\u2010Ficoll revealed that, in contrast to NTG B cell responses, TG B cell responses could not be enhanced by co\u2010culture with T cells. However, soluble T cell factor enhancement of the TG B cell responses was normal. These data suggest that the mCD43 expression on B cells may inhibit cell interactions that are important for enhanced TI Ag responses. The anti\u2010adhesive forces of mucins in general may thus be critical in regulating both TD and TI humoral responses.<\/jats:p>","DOI":"10.1002\/eji.1830270906","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T05:33:26Z","timestamp":1172813606000},"page":"2152-2159","source":"Crossref","is-referenced-by-count":2,"title":["Expression of mouse CD43 in the B cell lineage of transgenic mice causes impaired immune responses to T\u2010independent antigens"],"prefix":"10.1002","volume":"27","author":[{"given":"Julie R.","family":"Ostberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard L.","family":"Dragone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melinda A.","family":"Borrello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard P.","family":"Phipps","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard K.","family":"Barth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G.","family":"Frelinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/1.4.347"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00191883"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00364226"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2700569"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.4.1328"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.16.4932"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200424"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02747.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1991.tb03751.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1182\/blood.V71.5.1226.1226","volume":"71","author":"Bettaieb A.","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"3751","DOI":"10.4049\/jimmunol.140.11.3751","volume":"140","author":"Gulley M. L.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_14_2","first-page":"335","volume":"64","author":"Fukuda M.","year":"1986","journal-title":"Med. Biol."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-74974-2_3"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.5.1213"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1182\/blood.V70.1.104.104","volume":"70","author":"Remold O. D. E.","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1988.tb01476.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.3792"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830211222"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"12779","DOI":"10.1016\/S0021-9258(18)67161-3","volume":"261","author":"Carlsson S. R.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"762","DOI":"10.4049\/jimmunol.131.2.762","volume":"141","author":"Fox R. I.","year":"1983","journal-title":"J. Immunol."},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68157-8"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.1.259"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"4194","DOI":"10.4049\/jimmunol.142.12.4194","volume":"142","author":"Silverman L. B.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1988.tb02399.x"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/350706a0"},{"issue":"141","key":"e_1_2_1_28_2","first-page":"2912","volume":"198","author":"Axelsson B.","journal-title":"J. Immunol."},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/1.4.347"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1182\/blood.V87.4.1272.bloodjournal8741272","volume":"87","author":"Bazil Ev.","year":"1996","journal-title":"Blood"},{"key":"e_1_2_1_31_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1084\/jem.182.1.139","volume":"182","author":"Sperling A. I.","year":"1995","journal-title":"J. Exp. Med."},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"4308","DOI":"10.4049\/jimmunol.140.12.4308","volume":"140","author":"Chatila T. A.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1989.tb02461.x"},{"key":"e_1_2_1_34_2","doi-asserted-by":"crossref","first-page":"4194","DOI":"10.1182\/blood.V86.11.4194.bloodjournal86114194","volume":"86","author":"Manjunath N.","year":"1996","journal-title":"Blood"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27564"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb08022.x"},{"key":"e_1_2_1_37_2","first-page":"46","volume":"79","author":"De Smet W.","year":"1993","journal-title":"Immunology"},{"key":"e_1_2_1_38_2","first-page":"431","volume":"80","author":"Rosenkranz A. R.","year":"1993","journal-title":"Immunology"},{"key":"e_1_2_1_39_2","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1182\/blood.V86.6.2228.bloodjournal8662228","volume":"86","author":"Sanchez\u2010Mateos P.","year":"1995","journal-title":"Blood"},{"key":"e_1_2_1_40_2","first-page":"638","volume":"82","author":"Weber S.","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)05403-F"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/354233a0"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.1.243"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42275-7"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.3.877"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.5.1769"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.8.1493"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90315-Z"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.11.5001"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.2.626"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/377535a0"},{"key":"e_1_2_1_52_2","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1182\/blood.V86.6.2302.bloodjournal8662302","volume":"86","author":"Giordanengo V.","year":"1995","journal-title":"Blood"},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.4.1151"},{"key":"e_1_2_1_54_2","first-page":"41","volume":"2","author":"Ostberg J. R.","year":"1996","journal-title":"Transgenics"},{"key":"e_1_2_1_55_2","first-page":"919","volume":"7","author":"Misawa Y.","year":"1992","journal-title":"Oncogens"},{"key":"e_1_2_1_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.23.13688"},{"key":"e_1_2_1_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.2.626"},{"key":"e_1_2_1_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(87)90135-4"},{"key":"e_1_2_1_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1987.tb02281.x"},{"key":"e_1_2_1_60_2","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.4049\/jimmunol.157.11.4876","volume":"157","author":"Ostberg J. R.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_1_61_2","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.4049\/jimmunol.127.6.2262","volume":"127","author":"Kincade P. W.","year":"1981","journal-title":"J. Immunol."},{"key":"e_1_2_1_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(80)90335-4"},{"key":"e_1_2_1_63_2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.4049\/jimmunol.127.3.873","volume":"127","author":"Kung J. T.","year":"1981","journal-title":"J. Immunol."},{"key":"e_1_2_1_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00741.x"},{"key":"e_1_2_1_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(88)90018-4"},{"key":"e_1_2_1_66_2","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.4049\/jimmunol.140.11.4003","volume":"140","author":"Bahler D. W.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(95)80109-X"},{"key":"e_1_2_1_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90334-K"},{"key":"e_1_2_1_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(85)90060-4"},{"key":"e_1_2_1_70_2","first-page":"75","volume":"78","author":"Wismans P. J.","year":"1989","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_71_2","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1128\/iai.60.2.503-509.1992","volume":"60","author":"Pecquet S. S.","year":"1992","journal-title":"Infect. Immun."},{"key":"e_1_2_1_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170414"},{"key":"e_1_2_1_73_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.4049\/jimmunol.146.1.327","volume":"146","author":"Su S. D.","year":"1991","journal-title":"J. Immunol"},{"key":"e_1_2_1_74_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.003255"},{"key":"e_1_2_1_75_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.002441"},{"key":"e_1_2_1_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/1.6.576"},{"key":"e_1_2_1_77_2","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.4049\/jimmunol.153.12.5503","volume":"153","author":"Wells S. M.","year":"1994","journal-title":"J. Immunol."},{"key":"e_1_2_1_78_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.3.857"},{"key":"e_1_2_1_79_2","doi-asserted-by":"crossref","first-page":"633","DOI":"10.4049\/jimmunol.131.2.633","volume":"131","author":"Mond J. J.","year":"1983","journal-title":"J. Immunol."},{"key":"e_1_2_1_80_2","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.4049\/jimmunol.125.3.1066","volume":"125","author":"Mond J. J.","year":"1980","journal-title":"J. Immunol."},{"key":"e_1_2_1_81_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.8.5113"},{"key":"e_1_2_1_82_2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.4049\/jimmunol.129.2.557","volume":"129","author":"Nordin A. A.","year":"1982","journal-title":"J. Immunol."},{"key":"e_1_2_1_83_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830161123"},{"key":"e_1_2_1_84_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.4.1012"},{"key":"e_1_2_1_85_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(84)90099-6"},{"key":"e_1_2_1_86_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5294.1906"},{"key":"e_1_2_1_87_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90111-3"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830270906","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830270906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T23:56:33Z","timestamp":1698450993000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830270906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":86,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1002\/eji.1830270906"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830270906","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}