{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T14:41:02Z","timestamp":1698504062786},"reference-count":16,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":3013,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Major regulators of programmed cell death, or apoptosis, are the members of the bcl\u20102 gene family. Recently, we reported that surface(s) IgM triggering of the human B lymphoma cell line BL\u201041 led to strong induction of bax\u2010\u03b1, a death\u2010promoting member of the bcl\u20102 family, and subsequently to induction of apoptosis, suggesting a potential regulatory role of bax\u2010\u03b1 in sIgM\u2010mediated cell death. In contrast, apoptosis\u2010resistant subclones of this cell line showed only weak bax\u2010\u03b1 expression, which was not inducible by sIgM cross\u2010linking. In this study, we were able to demonstrate the functional significance of this observation. We stably transfected bax\u2010\u03b1 into a BL\u201041 subline resistant against sIgM\u2010mediated apoptosis. Several bax\u2010\u03b1 overexpressing clones could be selected, which all showed enhanced sensitivity for sIgM\u2010mediated apoptosis. In contrast, no sensitive clone could be identified in a large number of mock controls. This clearly indicates that induction of bax\u2010\u03b1 is a critical regulatory step, which sensitizes B cells for sIgM\u2010mediated apoptosis.<\/jats:p>","DOI":"10.1002\/eji.1830270947","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T05:33:26Z","timestamp":1172813606000},"page":"2466-2468","source":"Crossref","is-referenced-by-count":8,"title":["Overexpression of the death\u2010promoting gene <i>bax<\/i>\u2010\u03b1 sensitizes human BL\u201041 Burkitt lymphoma cells for surface IgM\u2010mediated apoptosis"],"prefix":"10.1002","volume":"27","author":[{"given":"Pamela","family":"Weinmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Bommert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus Y.","family":"Mapara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"D\u00f6rken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf C.","family":"Bargou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(94)90102-3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90060-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830211044"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200810"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","unstructured":"Hartley S. 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