{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:14:40Z","timestamp":1770718480759,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,12,5]],"date-time":"2005-12-05T00:00:00Z","timestamp":1133740800000},"content-version":"vor","delay-in-days":2987,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the protective functions of macrophages and \u03b3\u03b4 T cells in adult CD45 exon 6\u2010deficient (CD45\u2212\/\u2212) mice against an intraperitoneal (i.p.) infection with <jats:italic>Listeria monocytogenes<\/jats:italic>. \u03b3\u03b4 T cells are preferentially localized in the spleen, liver, and intraperitoneal cavity of the adult CD45\u2212\/\u2212 mice. Increased numbers of \u03b3\u03b4 T cells were observed after i.p. infection with <jats:italic>L. monocytogenes<\/jats:italic> in the peritoneal cavity of C57BL\/6 (CD45+\/+) mice but not in CD45\u2212\/\u2212 mice. The \u03b3\u03b4 T cells showed predominant usage of V\u03b45 and V\u03b46 rearranged to J\u03b41 in the infected CD45\u2212\/\u2212 mice which are the same as those used by resident \u03b3\u03b4 T cells of noninfected CD45+\/+ and CD45\u2212\/\u2212 mice. Furthermore, we analyzed the protective abilities of the CD45\u2212\/\u2212, CD45+\/+, and \u03b3\u03b4 T cell\u2010depleted mice at the early stage of the listerial infection. The numbers of bacteria in the spleens and livers of the CD45\u2212\/\u2212 mice 5 days after the listerial infection were almost ten times larger than those in the CD45\u2212\/\u2212 and \u03b3\u03b4 T cell\u2010depleted CD45+\/+ mice. Macrophages showed normal antigen presentation, nitric oxide production and bactericidal activity for <jats:italic>L. monocytogenes<\/jats:italic> despite their lacking CD45 surface expression, suggesting that CD45\u2010negative macrophages have a minimal influence on the increased bacterial multiplication in the CD45\u2212\/\u2212 mice. These results suggest that the \u03b3\u03b4 T cells are induced by the bacterial infection in a CD45\u2010dependent manner, and that unresponsiveness of the \u03b3\u03b4 T cells results in only weak protection against <jats:italic>L. monocytogenes<\/jats:italic> in CD45\u2212\/\u2212 mice.<\/jats:p>","DOI":"10.1002\/eji.1830271013","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T04:08:42Z","timestamp":1172808522000},"page":"2549-2556","source":"Crossref","is-referenced-by-count":3,"title":["Normal macrophage functions, but impaired induction of \u03b3\u03b4 T cells, at the site of bacterial infection in CD45 exon 6\u2010deficient mice"],"prefix":"10.1002","volume":"27","author":[{"given":"Shigeru","family":"Fujise","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Kishihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung\u2010Yil","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goro","family":"Matsuzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kikuo","family":"Nomoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"3718","DOI":"10.4049\/jimmunol.139.11.3718","volume":"139","author":"Lefrancois L.","year":"1987","journal-title":"J. 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