{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:57:11Z","timestamp":1758477431107},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,2,24]],"date-time":"2003-02-24T00:00:00Z","timestamp":1046044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent progress has begun to define the interactions and signaling pathways that are triggered during positive selection. To identify and further examine self\u2010peptides that can mediate positive selection, we searched a protein\u2010database to find peptides that have minimal homology with the viral peptide (p33) that activates a defined P14 transgenic TCR. We identified four peptides that could bind the restriction element H\u20102D<jats:sup>b<\/jats:sup> and induce proliferation of P14 transgenic splenocytes at high concentration. Two of the four peptides (DBM and RPP) were able to positively select thevirus\u2010specific TCR in fetal thymic organ culture but were unable to induce clonal deletion. Reverse\u2010phase HPLC and mass spectrometry demonstrated that these peptides were presented by H\u20102D<jats:sup>b<\/jats:sup> molecules on thymic epithelial cell lines. We also examined whether the selecting ligands altered T cell responsiveness <jats:italic>in vitro<\/jats:italic>. DBM\u2010selected T cells lost their ability to respond to the positively selecting ligand DBM, whereas RPP\u2010selected T cells only retainrd their ability to respond to high concentrations of RPP. These results demonstrate that self\u2010peptides that mediate positiveselection can differentially \"tune\" the activation threshold of T cells and alter the functional repertoire of T cells.<\/jats:p>","DOI":"10.1002\/eji.200323143","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T23:11:00Z","timestamp":1048029060000},"page":"685-696","source":"Crossref","is-referenced-by-count":14,"title":["Weak agonist self\u2010peptides promote selection and tuning of virus\u2010specific T 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