{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:29:02Z","timestamp":1779107342716,"version":"3.51.4"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T00:00:00Z","timestamp":1047945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    To analyze how combinatorial light (L) chain diversity influences the B cell repertoire, we studied mice with a homozygous immunoglobulin\u2010heavy\u2010chain null mutation (\u03bcMT), in which the B cell developmental block was overridden by the expression of a transgenic immunoglobulin \u03bc heavy (H) chain derived from a vesicular stomatitis virus Indiana serotype (VSV\u2010IND)\u2010neutralizing Ab (T11\u03bcMT mice). The randomly integrated transgene could not undergo secondary rearrangements and was expressed in combination with endogenous \u03ba or \u03bb chains. T11\u03bcMT mice had a skewed B cell repertoire as evidenced by 30\u201360% VSV\u2010IND\u2010specific peripheral B cells and spontaneous VSV\u2010IND\u2010neutralizing serum titers. Upon immunization, T11\u03bcMT mice mounted specific IgM antibody responses against VSV\u2010IND but, interestingly, they also responded against VSV New Jersey serotype (VSV\u2010NJ), lymphocytic choriomeningitis virus, poliovirus and\n                    <jats:italic>Salmonella typhi<\/jats:italic>\n                    porins. Variable\u2010region sequence analysis revealed that VSV\u2010NJ\u2010specific antibodies expressed numerous L chains in combination with the transgenic H chain, which was devoid of hypermutations. Thus, in T11\u03bcMT mice combinatorial L chain variability alone is able to build up a sufficiently complex B cell repertoire to mount protective immunoglobulin responses against a variety of pathogens.\n                  <\/jats:p>","DOI":"10.1002\/eji.200323340","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T08:58:47Z","timestamp":1048841927000},"page":"950-961","source":"Crossref","is-referenced-by-count":22,"title":["Combinatorial immunoglobulin light chain variability creates sufficient B cell diversity to mount protective antibody responses against pathogen infections"],"prefix":"10.1002","volume":"33","author":[{"given":"Beatrice\u2004M.","family":"Senn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantino","family":"L\u00f3pez\u2010Mac\u00edas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Kalinke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Lamarre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Isibasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf\u2004M.","family":"Zinkernagel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Hengartner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,18]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/302575a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.13.4118"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8356452"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.495"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60450-2"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/381751a0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.999"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/28419"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.4.765"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.1009"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60909-8"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.3.375"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(95)80010-7"},{"key":"e_1_2_7_14_2","first-page":"75","article-title":"Immunogenicity and antigen structure. 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