{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T15:03:38Z","timestamp":1771945418150,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,3,24]],"date-time":"2003-03-24T00:00:00Z","timestamp":1048464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Disseminated infections by the opportunistic yeast <jats:italic>Cryptococcus neoformans<\/jats:italic> are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of GXM uptake on phagocytic cell function. GXM was efficiently bound and internalized by both types of phagocytic cells, with maximal loading at 50\u2004\u03bcg\/ml, a GXM concentration found in serum and cerebrospinal fluid of some cryptococcosis patients. However, substantial differences were noted in the kinetics for uptake by macrophages and neutrophils. Whereas neutrophils rapidly ingested limited amounts of GXM and then expelled or degraded it after 1\u2004h of incubation, macrophages demonstrated continuous intracellular accumulation for up to 1\u2004week of incubation. Accumulation of GXM by neutrophils was accompanied by reduced anticryptococcal activity, suggesting one more mechanism for virulence enhancement by the major capsular component of <jats:italic>C.<\/jats:italic>\u2004<jats:italic>neoformans.<\/jats:italic><\/jats:p>","DOI":"10.1002\/eji.200323388","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T13:58:47Z","timestamp":1048859927000},"page":"1041-1051","source":"Crossref","is-referenced-by-count":35,"title":["Differences in outcome of the interaction between <i>Cryptococcus neoformans<\/i> glucuronoxylomannan and human monocytes and neutrophils"],"prefix":"10.1002","volume":"33","author":[{"given":"Claudia","family":"Monari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia","family":"Retini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale","family":"Netski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Bistoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas\u2004R.","family":"Kozel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Vecchiarelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,24]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.38.6.407.417"},{"key":"e_1_2_7_2_2","first-page":"145","article-title":"Detection of cryptococcal polysaccharide in serum and spinal fluid: value in diagnosis and prognosis.","volume":"77","author":"Bennett J. 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