{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:55:00Z","timestamp":1790373300158,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,2,24]],"date-time":"2003-02-24T00:00:00Z","timestamp":1046044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The causative agents of Lyme disease,<jats:italic> Borrelia burgdorferi <\/jats:italic>s.s., <jats:italic>B.\u2004garinii<\/jats:italic>, and <jats:italic>B.\u2004afzelii<\/jats:italic>, differ in their susceptibility to complement\u2010mediated lysis. This phenomenon apparently depends on the expression of proteins termed complement regulator\u2010acquiring surface proteins (CRASP) and their binding to the inhibitory plasma proteins factor H and FHL\u20101. To characterize these bacterial proteins in more detail we have now isolated from a <jats:italic>B.\u2004burgdorferi<\/jats:italic> expression library a novel factor H\u2010binding protein. In accordance with our previous studies this protein was termed BbCRASP\u20103 and represents a novel member of the polymorphic Erp (OspE\/F\u2010related) protein family. On the basis of protease accessibility assays using intact spirochetes, BbCRASP\u20103 isidentified as a surface\u2010exposed protein and binds the C\u2010terminal short consensus repeats of factor H. Applying deletion mutants of BbCRASP\u20103, the factor H\u2010binding site was mapped to the nine\u2010amino\u2010acid motif LEVLKKNLK localized at the C\u2010terminal end of BbCRASP\u20103. Factor H bound to BbCRASP\u20103 maintains its cofactor activity in factor I\u2010mediated C3b inactivation. Binding of BbCRASP\u20103 to factor H can be inhibited by heparin, a physiological ligand of the complement regulator factor H. Blocking of factor\u2010H\u2010binding by soluble BbCRASP\u20103 leads to an increase of complement deposition on intermediate serum\u2010resistant strain ZS7. In conclusion, BbCRASP\u20103 has been identified as a novel factor H\u2010binding protein on <jats:italic>B.\u2004burgdorferi<\/jats:italic> which by conferring complement resistance to the pathogen may contribute to its persistence in the mammalian host.<\/jats:p>","DOI":"10.1002\/eji.200323571","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T23:11:00Z","timestamp":1048029060000},"page":"697-707","source":"Crossref","is-referenced-by-count":111,"title":["Immune evasion of <i>Borrelia burgdorferi<\/i>: mapping of a complement\u2010inhibitor factor H\u2010binding site of BbCRASP\u20103, a novel member of the Erp protein family"],"prefix":"10.1002","volume":"33","author":[{"given":"Peter","family":"Kraiczy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Hellwage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Skerka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kirschfink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Brade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u2004F.","family":"Zipfel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Wallich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,2,24]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200104053441406"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(99)00049-8"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01432-7"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-3109(99)00015-6"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198908313210906"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.633"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02298-3"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(11)80087-2"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004300050038"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1128\/iai.65.4.1228-1236.1997","article-title":"Complement\u2010mediated serum sensitivity among spirochetes that cause Lyme disease.","volume":"65","author":"Van Dam A. 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