{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:34:42Z","timestamp":1761154482329},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,6,13]],"date-time":"2003-06-13T00:00:00Z","timestamp":1055462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the effect of <jats:italic>Borrelia burgdorferi<\/jats:italic> lipoproteins (outer surface protein\u2004A) and the synthetic lipohexapeptide tripalmitoyl\u2010S\u2010glyceryl\u2010Cys\u2010Ser\u20104(Lys) (Pam<jats:sub>3<\/jats:sub>\u2010Cys) on isolated lymph node (LN) cells from Lyme disease\u2010susceptible (C3H\/HeJ) and \u2010resistant (C57BL\/6J) mice. Mice were either infected with <jats:italic>B.\u2004burgdorferi<\/jats:italic> for 1\u2004week or left uninfected. Lipoprotein\u2010stimulated LN cells from infected C3H\/HeJ mice produced significantly higher levels of the inflammatory cytokines IL\u20106 and IFN\u2010\u03b3 than did cells from C57BL\/6J mice. Cells from uninfected mice did not respond. No TNF\u2010\u03b1 or IL\u20101\u03b2 were produced by LN cells from infected mice of either strain in response to lipoprotein or <jats:italic>B.\u2004burgdorferi <\/jats:italic>spirochetes. Unlike with IL\u20106 or IFN\u2010\u03b3, LN cells from either strain failed to produce IL\u201010 in response to lipoproteins. However, the LN cells were able to produce this cytokine in response to <jats:italic>B.\u2004burgdorferi<\/jats:italic> spirochetes or after incubation with phorbol\u201012\u2010myristate\u201013\u2010acetate\/ionomycin, anti\u2010CD3 antibody alone or anti\u2010CD3 combined with anti\u2010CD28 antibodies. Addition of exogenous IL\u201010 to lipopeptide\u2010stimulated cultures significantly reduced IFN\u2010\u03b3 and IL\u20106 production in a dose\u2010dependent fashion. This inhibition was more effective with cells from disease\u2010resistant C57BL\/6J mice than with cells from disease\u2010susceptible C3H\/HeJ mice. The proclivity to disease of the C3H\/HeJ mouse could be simultaneously based on the phenomena of enhanced inflammatory responsiveness to lipoproteins and diminished ability to respond to IL\u201010. An investigation of the determinants of these two phenomena could be used as a blueprint to elucidate the pathogenesis of Lyme disease in humans.<\/jats:p>","DOI":"10.1002\/eji.200323655","type":"journal-article","created":{"date-parts":[[2003,6,20]],"date-time":"2003-06-20T11:13:35Z","timestamp":1056107615000},"page":"1934-1940","source":"Crossref","is-referenced-by-count":17,"title":["Differential acquired immune responsiveness to bacterial lipoproteins in Lyme disease\u2010resistant and \u2010susceptible mouse strains"],"prefix":"10.1002","volume":"33","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Ganapamo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vida\u2004A.","family":"Dennis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario\u2004T.","family":"Philipp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,6,13]]},"reference":[{"key":"e_1_2_7_1_2","first-page":"22","article-title":"Lyme borreliosis in the laboratory mice.","volume":"3","author":"Barthold S. 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