{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:05:39Z","timestamp":1790892339139,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T00:00:00Z","timestamp":1053993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Signal transducer and activator of transcription 6 (Stat6) has a crucial role in regulation of IL\u20104\u2010induced gene responses. Stat6\u2010binding sites are present in the promoters of both ubiquitously and cell\u2010type\u2010specifically expressed genes. The promoter regions of IL\u20104\u2010inducible genes contain <jats:italic>cis<\/jats:italic>\u2010acting elements for several transcription factors that act in concert with Stat6 and are also likely to modulate lineage\u2010specific gene expression. We have observed that the Stat6 response element from the B\u2010cell\u2010specific Ig\u03f5 promoter is readily activated upon IL\u20104 stimulation in B cells but not in non\u2010hematopoietic cells. A minimal low\u2010affinity PU.1\u2010core\u2010binding sequence (5\u2032\u2010AGAA\u20103\u2032) was identified within the Stat6 DNA\u2010binding site in the Ig\u03f5 promoter. Ectopic expression of the myeloid\u2010 and B\u2010cell\u2010specific transcription factor PU.1 restored the IL\u20104\u2010inducibility of the Ig\u03f5\u2010Stat6 response element in HepG2 cells, and the induction required an intact PU.1\u2010binding sequence. Both the transactivation and the DNA\u2010binding domains of PU.1 were required for induction of Stat6\u2010mediated transcription. The co\u2010operation between PU.1 and Stat6 in transactivation of the Ig\u03f5 gene represents a molecular mechanism for the fine\u2010tuning of cell\u2010type\u2010restricted expression of IL\u20104\u2010induced gene responses.<\/jats:p>","DOI":"10.1002\/eji.200323680","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T16:03:22Z","timestamp":1054051402000},"page":"1727-1735","source":"Crossref","is-referenced-by-count":19,"title":["PU.1 is required for transcriptional activation of the Stat6 response element in the Ig\u03f5 promoter"],"prefix":"10.1002","volume":"33","author":[{"given":"Marko","family":"Pesu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saara","family":"Aittom\u00e4ki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuuli","family":"V\u00e4lineva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli","family":"Silvennoinen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,5,27]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.701"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.41.25555"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"5637","DOI":"10.4049\/jimmunol.155.12.5637","article-title":"Induction of germ\u2010line gamma 1 and epsilon Ig gene expression in murine B cells. IL\u20104 and the CD40 ligand\u2010CD40 interaction provide distinct but synergistic signals.","volume":"155","author":"Warren W. D.","year":"1995","journal-title":"J.\u2004Immunol."},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1948049"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/380630a0"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.4049\/jimmunol.158.1.263","article-title":"Analysis of the promoter elements necessary for IL\u20104 and anti\u2010CD40 antibody induction of\nmurine Fc epsilon RII (CD23): comparison with the germline epsilon promoter.","volume":"158","author":"Richards M. 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