{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:39:14Z","timestamp":1779277154135,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,3,27]],"date-time":"2003-03-27T00:00:00Z","timestamp":1048723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro\u2010) and precursor (pre\u2010) B\u2004cells. As part of the pre\u2010B\u2004cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B\u2004lymphocyte development. Using cell lines, we identified a lineage\u2010 and developmental\u2010stage\u2010specific VpreB1 enhancer. Here, we analyze its specificity <jats:italic>in vivo<\/jats:italic> by generating transgenic mice in which expression of a reporter gene (human CD122) is regulated by the VpreB1 enhancer in the context of its own promoter. All transgenic lines expressed thereporter gene in the bone marrow in a copy number\u2010independent manner, whereas expression levels were integration site\u2010dependent. While the enhancer is not tissue specific, within the B\u2004cell lineage the expression pattern of human CD122 mimicked that of endogenous VpreB1. Thus, low levels were detected in pro\u2010B\u2004cells, high levels in pre\u2010BI and slightly lower levels in pre\u2010BII cells; no expression was detected in immature\/mature B\u2004cells. Furthermore, when <jats:italic>in vitro<\/jats:italic> cultured transgenic pre\u2010B\u2004cells differentiated into immature B\u2004cells there was concomitant down\u2010regulation of human CD122 andendogenous VpreB1. Thus the VpreB1 enhancer is sufficient to ensure developmental stage\u2010specific expression of a reporter gene in B\u2004lymphocytes <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/eji.200323702","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T13:58:47Z","timestamp":1048859927000},"page":"1117-1126","source":"Crossref","is-referenced-by-count":3,"title":["The VpreB1 enhancer drives developmental stage\u2010specific gene expression <i>in vivo<\/i>"],"prefix":"10.1002","volume":"33","author":[{"given":"Steve","family":"Licence","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Persson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Mundt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inga\u2010Lill","family":"M\u00e5rtensson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,27]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5323(02)00066-0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/350423a0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90293-L"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.4.435"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.12.6286"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.1002\/j.1460-2075.1987.tb02500.x","article-title":"A second gene, VpreB in the lambda\u20045 locus of the mouse, which appears to be selectively expressed in pre\u2010B\u2004lymphocytes.","volume":"6","author":"Kudo A.","year":"1987","journal-title":"EMBO J."},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/324579a0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260428"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.6.863"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220106"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270145"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199803)28:03<787::AID-IMMU787>3.0.CO;2-H"},{"key":"e_1_2_7_13_2","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.4049\/jimmunol.155.5.2498","article-title":"Identification and localization of a developmentalstage\u2010specific promoter activity from the murine lambda\u20045 gene.","volume":"155","author":"Yang J.","year":"1995","journal-title":"J. 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